农杆菌介导的基因转移主要导致转基因植物以单孟德尔因子的形式传递 T-DNA。
Agrobacterium-Mediated Gene Transfer Results Mainly in Transgenic Plants Transmitting T-DNA as a Single Mendelian Factor.
机构信息
Laboratorium voor Genetische Virologie, Vrije Universiteit Brussel, B-1640 Sint-Genesius-Rode, Belgium.
出版信息
Genetics. 1986 Sep;114(1):303-13. doi: 10.1093/genetics/114.1.303.
Abstract
Forty-four independent transformed tobacco plants were obtained from a cocultivation experiment with Agrobacterium tumefaciens strains carrying modified Ti-plasmids. The transformed plants were either self-fertilized or crossed with nontransformed plants or with other transformed plants. The segregation of a phenotypic marker (kanamycin resistance) in the progenies of these plants was determined. In 40 cases out of 44, the segregation of the kanamycin resistance marker is consistent with Mendelian genetics. Among these 40 clones, 35 contain a single kanamycin resistance locus. The five others segregate two independent resistance loci. In two of the single insert clones, the segregation ratio after selfing indicates that the T-DNA insertion may have caused a recessive lethal mutation.
摘要
从携带修饰过的 Ti 质粒的根瘤农杆菌菌株的共培养实验中，获得了 44 株独立的转化烟草植株。转化植株进行自交或与非转化植株或其他转化植株杂交。这些植株后代中表型标记（卡那霉素抗性）的分离情况得到了确定。在 44 个案例中，有 40 个卡那霉素抗性标记的分离符合孟德尔遗传学。在这 40 个克隆中，有 35 个含有单个卡那霉素抗性基因座。其余 5 个分离出两个独立的抗性基因座。在两个单插入克隆中，自交后的分离比表明 T-DNA 的插入可能导致了隐性致死突变。
相似文献
1
Agrobacterium-Mediated Gene Transfer Results Mainly in Transgenic Plants Transmitting T-DNA as a Single Mendelian Factor.农杆菌介导的基因转移主要导致转基因植物以单孟德尔因子的形式传递 T-DNA。
Genetics. 1986 Sep;114(1):303-13. doi: 10.1093/genetics/114.1.303.
2
Small high-yielding binary Ti vectors pLSU with co-directional replicons for Agrobacterium tumefaciens-mediated transformation of higher plants.带有同向复制子的小型高产二元 Ti 载体 pLSU ，用于根癌农杆菌介导的高等植物转化。
Plant Sci. 2012 May;187:49-58. doi: 10.1016/j.plantsci.2012.01.012. Epub 2012 Feb 2.
3
Mendelian transmission of genes introduced into plants by the Ti plasmids of Agrobacterium tumefaciens.根癌土壤杆菌的Ti质粒导入植物的基因的孟德尔式传递。
Mol Gen Genet. 1981;183(2):209-13. doi: 10.1007/BF00270619.
4
High efficiency transgene segregation in co-transformed maize plants using an Agrobacterium tumefaciens 2 T-DNA binary system.利用根癌农杆菌双T-DNA二元系统在共转化玉米植株中实现高效转基因分离
Transgenic Res. 2002 Aug;11(4):381-96. doi: 10.1023/a:1016390621482.
5
Efficient octopine Ti plasmid-derived vectors for Agrobacterium-mediated gene transfer to plants.用于农杆菌介导的基因向植物转移的高效章鱼碱Ti质粒衍生载体。
Nucleic Acids Res. 1985 Jul 11;13(13):4777-88. doi: 10.1093/nar/13.13.4777.
6
Expression and inheritance of kanamycin resistance in a large number of transgenic petunias generated by Agrobacterium-mediated transformation.利用农杆菌介导转化生成的大量转基因矮牵牛中卡那霉素抗性的表达和遗传。
Plant Mol Biol. 1988 May;11(3):355-64. doi: 10.1007/BF00027392.
7
Transgenic Medicago truncatula plants obtained from Agrobacterium tumefaciens -transformed roots and Agrobacterium rhizogenes-transformed hairy roots.通过根癌农杆菌转化的根和发根农杆菌转化的毛状根获得的转基因蒺藜苜蓿植株。
Planta. 2006 May;223(6):1344-54. doi: 10.1007/s00425-006-0268-2. Epub 2006 Mar 31.
8
Transformation and regeneration of the self-incompatible species Nicotiana alata Link & Otto.自交不亲和物种烟草（Nicotiana alata Link & Otto）的转化与再生
Plant Mol Biol. 1990 May;14(5):815-24. doi: 10.1007/BF00016514.
9
pSa causes oncogenic suppression of Agrobacterium by inhibiting VirE2 protein export.pSa通过抑制VirE2蛋白输出对根癌土壤杆菌产生致癌抑制作用。
J Bacteriol. 1999 Jan;181(1):186-96. doi: 10.1128/JB.181.1.186-196.1999.
10
Studies of the structure and functional organization of foreign DNA integrated into the genome of Nicotiana tabacum.对整合到烟草基因组中的外源DNA的结构和功能组织的研究。
DNA. 1986 Dec;5(6):473-82. doi: 10.1089/dna.1.1986.5.473.
引用本文的文献
1
Conquering Limitations: Exploring the Factors that Drive Successful Agrobacterium-Mediated Genetic Transformation of Recalcitrant Plant Species.克服局限：探索驱动顽固植物物种农杆菌介导遗传转化成功的因素
Mol Biotechnol. 2024 Aug 23. doi: 10.1007/s12033-024-01247-x.
2
Engineering tolerance to CLCuD in transgenic Gossypium hirsutum cv. HS6 expressing Cotton leaf curl Multan virus-C4 intron hairpin.在表达棉花卷叶多尼病毒-C4 内含子发夹的转基因 Gossypium hirsutum cv. HS6 中工程耐受 CLCuD。
Sci Rep. 2021 Jul 8;11(1):14172. doi: 10.1038/s41598-021-93502-3.
3
A novel Ap2/ERF transcription factor from Stipa purpurea leads to enhanced drought tolerance in Arabidopsis thaliana.一种来自紫花针茅的新型Ap2/ERF转录因子可提高拟南芥的耐旱性。
Plant Cell Rep. 2016 Nov;35(11):2227-2239. doi: 10.1007/s00299-016-2030-y. Epub 2016 Jul 21.
4
Expression and inheritance of kanamycin resistance in a large number of transgenic petunias generated by Agrobacterium-mediated transformation.利用农杆菌介导转化生成的大量转基因矮牵牛中卡那霉素抗性的表达和遗传。
Plant Mol Biol. 1988 May;11(3):355-64. doi: 10.1007/BF00027392.
5
Genetic analysis of T-DNA insertions into the tobacco genome.烟草基因组中 T-DNA 插入的遗传分析。
Plant Cell Rep. 1988 Dec;7(7):571-4. doi: 10.1007/BF00272760.
6
Characterization of transgenic sulfonylurea-resistant flax (Linum usitatissimum).转磺酰脲类抗性亚麻（Linum usitatissimum）的特性分析。
Theor Appl Genet. 1992 Jul;84(3-4):480-6. doi: 10.1007/BF00229510.
7
Molecular tagging of the tobacco chromosome carrying the TMV-resistance gene (N gene) by Agrobacterium-mediated transformation.利用农杆菌介导转化对携带 TMV 抗性基因（N 基因）的烟草染色体进行分子标记。
Theor Appl Genet. 1992 May;83(8):981-6. doi: 10.1007/BF00232960.
8
Field performance of derived generations of transgenic tobacco.转基因烟草衍生世代的田间表现。
Theor Appl Genet. 1993 Aug;86(7):875-9. doi: 10.1007/BF00212615.
9
Expression and inheritance pattern of two foreign genes in petunia.矮牵牛中外源基因的表达和遗传模式。
Theor Appl Genet. 1994 Jun;88(3-4):433-40. doi: 10.1007/BF00223657.
10
Synthesis and protein body deposition of maize 15-kd zein in transgenic tobacco seeds.转基因烟草种子中玉米 15-kd 醇溶蛋白的合成和蛋白体沉积。
EMBO J. 1987 Nov;6(11):3213-21. doi: 10.1002/j.1460-2075.1987.tb02638.x.
本文引用的文献
1
Chimeric genes as dominant selectable markers in plant cells.嵌合基因作为植物细胞中的显性选择标记。
EMBO J. 1983;2(6):987-95. doi: 10.1002/j.1460-2075.1983.tb01532.x.
2
Site-specific mutagenesis of Agrobacterium Ti plasmids and transfer of genes to plant cells.根癌土壤杆菌Ti质粒的位点特异性诱变及基因向植物细胞的转移。
J Mol Appl Genet. 1981;1(2):149-64.
3
Mendelian transmission of genes introduced into plants by the Ti plasmids of Agrobacterium tumefaciens.根癌土壤杆菌的Ti质粒导入植物的基因的孟德尔式传递。
Mol Gen Genet. 1981;183(2):209-13. doi: 10.1007/BF00270619.
4
Expression of bacterial genes in plant cells.细菌基因在植物细胞中的表达。
Proc Natl Acad Sci U S A. 1983 Aug;80(15):4803-7. doi: 10.1073/pnas.80.15.4803.
5
Regeneration of intact tobacco plants containing full length copies of genetically engineered T-DNA, and transmission of T-DNA to R1 progeny.含有基因工程T-DNA全长拷贝的完整烟草植株的再生，以及T-DNA向R1代后代的传递。
Cell. 1983 Apr;32(4):1033-43. doi: 10.1016/0092-8674(83)90288-x.
6
Tumor DNA structure in plant cells transformed by A. tumefaciens.根癌农杆菌转化的植物细胞中的肿瘤DNA结构。
Science. 1980 Sep 19;209(4463):1385-91. doi: 10.1126/science.6251546.
7
The functional organization of the nopaline A. tumefaciens plasmid pTiC58.胭脂碱型根癌土壤杆菌质粒pTiC58的功能组织
Plasmid. 1980 Mar;3(2):212-30. doi: 10.1016/0147-619x(80)90110-9.
8
Streptomycin-resistant plants from callus culture of haploid tobacco.来自单倍体烟草愈伤组织培养的抗链霉素植物。
Nat New Biol. 1973 Jul 4;244(131):29-30. doi: 10.1038/newbio244029a0.
Zotero 插件