农杆菌介导的基因转移主要导致转基因植物以单孟德尔因子的形式传递 T-DNA。
Agrobacterium-Mediated Gene Transfer Results Mainly in Transgenic Plants Transmitting T-DNA as a Single Mendelian Factor.
机构信息
Laboratorium voor Genetische Virologie, Vrije Universiteit Brussel, B-1640 Sint-Genesius-Rode, Belgium.
出版信息
Genetics. 1986 Sep;114(1):303-13. doi: 10.1093/genetics/114.1.303.
Abstract
Forty-four independent transformed tobacco plants were obtained from a cocultivation experiment with Agrobacterium tumefaciens strains carrying modified Ti-plasmids. The transformed plants were either self-fertilized or crossed with nontransformed plants or with other transformed plants. The segregation of a phenotypic marker (kanamycin resistance) in the progenies of these plants was determined. In 40 cases out of 44, the segregation of the kanamycin resistance marker is consistent with Mendelian genetics. Among these 40 clones, 35 contain a single kanamycin resistance locus. The five others segregate two independent resistance loci. In two of the single insert clones, the segregation ratio after selfing indicates that the T-DNA insertion may have caused a recessive lethal mutation.
摘要
从携带修饰过的 Ti 质粒的根瘤农杆菌菌株的共培养实验中，获得了 44 株独立的转化烟草植株。转化植株进行自交或与非转化植株或其他转化植株杂交。这些植株后代中表型标记（卡那霉素抗性）的分离情况得到了确定。在 44 个案例中，有 40 个卡那霉素抗性标记的分离符合孟德尔遗传学。在这 40 个克隆中，有 35 个含有单个卡那霉素抗性基因座。其余 5 个分离出两个独立的抗性基因座。在两个单插入克隆中，自交后的分离比表明 T-DNA 的插入可能导致了隐性致死突变。
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