Aboofazeli Nafiseh, Khosravi Solmaz, Bagheri Hedayat, Chandler Stephen F, Pan Shen Q, Azadi Pejman
Novin Giti Gene Biotech R&D Center (N.G.G), Imam Khomeini Higher Education Center, Karaj, Iran.
Department of Genetic Engineering, Agricultural Research, Education and Extension Organization (AREEO), Agricultural Biotechnology Research Institute of Iran (ABRII), Postal Code: 3135933152, Karaj, Iran.
Mol Biotechnol. 2024 Aug 23. doi: 10.1007/s12033-024-01247-x.
Agrobacterium-mediated transformation is a preferred method for genetic engineering and genome editing of plants due to its numerous advantages, although not all species exhibit transformability. Genetic engineering and plant genome editing methods are technically challenging in recalcitrant crop plants. Factors affecting the poor rate of transformation in such species include host genotype, Agrobacterium genotype, type of explant, physiological condition of the explant, vector, selectable marker, inoculation method, chemical additives, antioxidative compounds, transformation-enhancing compounds, medium formulation, optimization of culture conditions, and pre-treatments. This review provides novel insights into the key factors involved in gene transfer facilitated by Agrobacterium and proposes potential solutions to overcome existing barriers to transformation in recalcitrant species, thereby contributing to improvement programs for these species. This review introduces the key factors that impact the effectiveness of a molecular breeding program using Agrobacterium-mediated transformation, specifically focusing on recalcitrant plant species.
由于农杆菌介导的转化具有诸多优点，它是植物基因工程和基因组编辑的首选方法，尽管并非所有物种都具有转化能力。基因工程和植物基因组编辑方法在顽拗作物中技术上具有挑战性。影响此类物种转化效率低下的因素包括宿主基因型、农杆菌基因型、外植体类型、外植体的生理状态、载体、选择标记、接种方法、化学添加剂、抗氧化化合物、转化增强化合物、培养基配方、培养条件优化和预处理。本综述对农杆菌介导的基因转移所涉及的关键因素提供了新的见解，并提出了克服顽拗物种现有转化障碍的潜在解决方案，从而有助于这些物种的改良计划。本综述介绍了影响使用农杆菌介导的转化进行分子育种计划有效性的关键因素，特别关注顽拗植物物种。
