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玉米(ZEA MAYS L.)核仁组织者区域:核糖体基因补偿或放大测试。

The Nucleolus Organizer Region of Maize (ZEA MAYS L.): Tests for Ribosomal Gene Compensation or Magnification.

机构信息

Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, Minnesota 55101 Department of Biological Sciences, Illinois State University, Normal, Illinois 61761.

出版信息

Genetics. 1974 Jun;77(2):285-97. doi: 10.1093/genetics/77.2.285.

Abstract

Ribosomal gene compensation and magnification that might be detected on a whole-plant basis was not found in maize. Plants monosomic for chromosome 6 (the NOR chromosome) were compared with monosomic-8 and monosomic-10 plants, disomic sibs, and parental lines. Assuming no rDNA compensation, monosomic-6 plants showed approximately the decrease expected in rRNA cistron number. Monosomic-8 had a normal ribosomal gene number, while monosomic-10 showed a decrease; but further documentation is needed. Besides demonstrating the absence of gene compensation, the results document our previous conclusion that maize chromosome 6 carries DNA complementary to ribosomal RNA. Further documentation was provided from studies with trisomic chromosome 6 plants showing proportional increases in ribosomal gene number. Progeny of the monosomic plants crossed as males to a standard singlecross hybrid possessed expected ribosomal gene numbers suggesting the lack of ribosomal gene magnification.-The ragged (rgd) mutant of maize, suspected of being deficient in rRNA cistrons, had a normal number.

摘要

在玉米中未发现可能在全株水平上检测到的核糖体基因补偿和扩增。比较了单体 6 号染色体(NOR 染色体)、单体 8 号和单体 10 号植物、二倍体姊妹系和亲本系。假设没有 rDNA 补偿,单体 6 号植物的 rRNA 顺式结构数量减少与预期相符。单体 8 号具有正常的核糖体基因数量,而单体 10 号则减少;但需要进一步的文件证明。除了证明没有基因补偿之外,这些结果还证明了我们之前的结论,即玉米 6 号染色体携带与核糖体 RNA 互补的 DNA。进一步的文件证明来自三体 6 号染色体植物的研究,这些植物的核糖体基因数量呈比例增加。单体植物的后代作为雄性与标准单交杂种杂交,具有预期的核糖体基因数量,表明没有核糖体基因扩增。玉米的粗糙(rgd)突变体,怀疑其 rRNA 顺式结构缺陷,数量正常。

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