Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, Minnesota 55101 Department of Biological Sciences, Illinois State University, Normal, Illinois 61761.
Genetics. 1974 Jun;77(2):285-97. doi: 10.1093/genetics/77.2.285.
Ribosomal gene compensation and magnification that might be detected on a whole-plant basis was not found in maize. Plants monosomic for chromosome 6 (the NOR chromosome) were compared with monosomic-8 and monosomic-10 plants, disomic sibs, and parental lines. Assuming no rDNA compensation, monosomic-6 plants showed approximately the decrease expected in rRNA cistron number. Monosomic-8 had a normal ribosomal gene number, while monosomic-10 showed a decrease; but further documentation is needed. Besides demonstrating the absence of gene compensation, the results document our previous conclusion that maize chromosome 6 carries DNA complementary to ribosomal RNA. Further documentation was provided from studies with trisomic chromosome 6 plants showing proportional increases in ribosomal gene number. Progeny of the monosomic plants crossed as males to a standard singlecross hybrid possessed expected ribosomal gene numbers suggesting the lack of ribosomal gene magnification.-The ragged (rgd) mutant of maize, suspected of being deficient in rRNA cistrons, had a normal number.
在玉米中未发现可能在全株水平上检测到的核糖体基因补偿和扩增。比较了单体 6 号染色体(NOR 染色体)、单体 8 号和单体 10 号植物、二倍体姊妹系和亲本系。假设没有 rDNA 补偿,单体 6 号植物的 rRNA 顺式结构数量减少与预期相符。单体 8 号具有正常的核糖体基因数量,而单体 10 号则减少;但需要进一步的文件证明。除了证明没有基因补偿之外,这些结果还证明了我们之前的结论,即玉米 6 号染色体携带与核糖体 RNA 互补的 DNA。进一步的文件证明来自三体 6 号染色体植物的研究,这些植物的核糖体基因数量呈比例增加。单体植物的后代作为雄性与标准单交杂种杂交,具有预期的核糖体基因数量,表明没有核糖体基因扩增。玉米的粗糙(rgd)突变体,怀疑其 rRNA 顺式结构缺陷,数量正常。