Department of Biology, University of Rochester, Rochester, New York 14627.
Genetics. 1977 Dec;87(4):717-42. doi: 10.1093/genetics/87.4.717.
The relationship between charge changes and electrophoretic mobility changes is investigated experimentally. The charge of several proteins is altered by reaction with small molecules of known structure and the change in electrophoretic mobility is measured. The method of Ferguson plots is used to separate charge and shape components of mobility differences. The average effect of an amino acid charge change on the mobility of the esterase-5( 1.00) allele of Drosophila pseudoobscura is estimated to be 0.046. This estimate is then used to apply the step model of Ohta and Kimura (1973) to electrophoretic mobility data for the esterase-5 locus of D. pseudoobscura and D. miranda. The variation in electrophoretic mobility at this locus was found to be in agreement with the predictions of the step model.
实验研究了电荷变化和电泳迁移率变化之间的关系。通过与已知结构的小分子反应来改变几种蛋白质的电荷，并测量电泳迁移率的变化。采用 Ferguson 图法将迁移率差异的电荷和形状分量分开。估计 Drosophila pseudoobscura 中酯酶-5( 1.00)等位基因的一个氨基酸电荷变化对迁移率的平均影响为 0.046。然后，利用 Ohta 和 Kimura（1973）的阶跃模型将该估计值应用于 D. pseudoobscura 和 D. miranda 酯酶-5 基因座的电泳迁移率数据。发现该基因座的电泳迁移率变化与阶跃模型的预测相符。
