拟暗果蝇、亲缘果蝇和拟菱果蝇酯酶-5基因座的比较研究。
A comparative study of the esterase-5 locus in Drosophila pseudoobscura, D. persimilis and D. miranda.
作者信息
Cobbs G, Prakash S
出版信息
Genetics. 1977 Apr;85(4):697-711. doi: 10.1093/genetics/85.4.697.
Electrophoretic phenotypes of the esterase-5 locus were examined in the sibling species D. pseudoobscura, D. persimilis and D. miranda. D. persimilis alleles were found to have uniformly higher charge on monomers than corresponding alleles of either D. pseudoobscura or D. miranda. Consequently, D. persimilis shares no alleles in common with either D. pseudoobscura or D. miranda, while the latter two species share a number of alleles. It was discovered that by increasing the concentration of acrylamide gel and increasing the length of migration, more allelic differences could be distinguished. Also more alleles were discovered by examining monomer mobility in addition to dimer mobility. In D. persimilis and D. miranda it was found that the previously known high frequency allelic classes broke down into several allelic classes. A test of goodness-of-fit to the infinite alleles model was done and a rough agreement with the model was found.
在亲缘物种拟暗果蝇（D. pseudoobscura）、黑腹果蝇（D. persimilis）和拟果蝇（D. miranda）中检测了酯酶 - 5位点的电泳表型。发现黑腹果蝇的等位基因在单体上的电荷比拟暗果蝇或拟果蝇的相应等位基因均匀更高。因此，黑腹果蝇与拟暗果蝇或拟果蝇没有共同的等位基因，而后两个物种共享一些等位基因。研究发现，通过增加丙烯酰胺凝胶的浓度和延长迁移长度，可以区分更多的等位基因差异。此外，除了二聚体迁移率外，通过检测单体迁移率还发现了更多等位基因。在黑腹果蝇和拟果蝇中，发现先前已知的高频等位基因类别分解为几个等位基因类别。对无限等位基因模型进行了拟合优度检验，发现与该模型大致相符。
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