Forest C L, Togasaki R K
Proc Natl Acad Sci U S A. 1975 Sep;72(9):3652-5. doi: 10.1073/pnas.72.9.3652.
A new technique is described for selection of temperature-sensitive mutants affecting gametogenesis in Chlamydomonas reinhardi. The first mutant found by this technique is characterized. Cells exhibiting the gam-1 phenotype are capable of sexual agglutination, but cannot form zygotes at the restrictive temperature. The mutation, however, has been expressed only in gametes of mating type (-). Cells of mating type (+) which carry this gene are able to engage in normal zygote formation. Temperature shift experiments and antibiotic studies have indicated that this gene is expressed within 6 hr after the onset of gametogenesis in liquid culture, and its product has a lifetime of about 4 hr at 35 degrees.
本文描述了一种用于筛选影响莱茵衣藻配子发生的温度敏感突变体的新技术。通过该技术发现的首个突变体得到了表征。表现出gam-1表型的细胞能够进行有性凝集,但在限制温度下无法形成合子。然而,该突变仅在交配型(-)的配子中表达。携带此基因的交配型(+)细胞能够正常形成合子。温度转换实验和抗生素研究表明,该基因在液体培养中配子发生开始后的6小时内表达,其产物在35摄氏度下的寿命约为4小时。