酿酒酵母中 HMa 和 HMalpha 基因座的 MAR1 调节因子。
MAR1-a Regulator of the HMa and HMalpha Loci in SACCHAROMYCES CEREVISIAE.
机构信息
Department of Genetics, University of California Berkeley, California 94720.
出版信息
Genetics. 1979 Sep;93(1):37-50. doi: 10.1093/genetics/93.1.37.
A mutation in the MAR1 (mating-type regulator) locus causing sterility in Saccharomyces cerevisiae is reported. The mutation maps on the left arm of linkage group IV between trp1 and cdc2 at a distance of about 27 cM from trp1 and about 31 cM from cdc2. Haploid strains with genotype MATalpha HMalpha HMa mar1-1 and MATa HMalpha HMamar1-1 are sterile. However, MATalpha hmalpha HMa mar1-1 and MATa HMalpha hma mar1-1 strains exhibit alpha and a mating type, respectively. The sterile strains can be "rare mated" with standard strains as a consequence of mutational changes at HMa and HMalpha. It is proposed that the MAR1 locus blocks the expression of MATalpha and MATa information thought to exist at HMa and HMalpha loci, respectively (Hicks, Strathern and Herskowitz, 1977). In a mar1-1 mutant, the expression of both HMalpha and HMa information leads to a nonmating phenotype similar to that of MATa/MATalpha diploids. The genetic evidence reported here is consistent with a central feature of the "cassette model", namely that HMalpha and hma carry MATa information and HMa and hmalpha carry MATalpha information.
报道了一个导致酿酒酵母不育的 MAR1（交配型调节）基因座突变。该突变位于连锁群 IV 的左臂上，在 trp1 和 cdc2 之间，距离 trp1 约 27 cM，距离 cdc2 约 31 cM。基因型为 MATalpha HMalpha HMa mar1-1 和 MATa HMalpha HMamar1-1 的单倍体菌株不育。然而，MATalpha hmalpha HMa mar1-1 和 MATa HMalpha hma mar1-1 菌株分别表现出α和 a 交配型。由于 HMa 和 HMalpha 发生突变，不育菌株可以与标准菌株“罕见交配”。据推测，MAR1 基因座阻止了分别存在于 HMa 和 HMalpha 基因座的 MATalpha 和 MATa 信息的表达（Hicks、Strathern 和 Herskowitz，1977）。在 mar1-1 突变体中，HMalpha 和 HMa 信息的表达都导致类似于 MATa/MATalpha 二倍体的非交配表型。这里报道的遗传证据与“盒式模型”的一个核心特征一致，即 HMalpha 和 hma 携带 MATa 信息，HMa 和 hmalpha 携带 MATalpha 信息。
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