Massas R, Servadio C, Sandbank U, Abramovici A
Laboratory of Developmental Pathology, Sackler School of Medicine, Tel-Aviv University, Israel.
Histol Histopathol. 1991 Apr;6(2):183-9.
Immunocytochemical characterization of several epithelial markers using the PAP technique was analyzed during different stages of induced prostatic hyperplasia in rats. Intact adolescent rats (42 days old) were treated with citral (3,7 dimethyl-2,6 octadienal) for 10, 30 and 100 days and their ventral prostate compared to untreated, matched-age animals. Among the epithelial markers studied the prostatic specific acid phosphatase was present in hyperplastic prostates of rats. The immunoreaction showed a fair correlation with the severity of lesion and duration of treatment. The prostatic specific antigen showed equally immunoreactive in both control and treated rats. The hyperplastic and normal rat prostates did not show immunoreactivity towards the other epithelial cell markers such as epithelial membrane antigen, carcinoembrionic antingen and alpha-fetoprotein antisera. It is concluded that prostatic specific acid phosphatase, and to a lesser extent prostatic specific antigen, might represent valuable markers for comparative studies of prostatic hyperplasia in rodents.
采用PAP技术对大鼠诱导性前列腺增生不同阶段的几种上皮标志物进行免疫细胞化学特征分析。将完整的青春期大鼠（42日龄）用柠檬醛（3,7 - 二甲基 - 2,6 - 辛二烯醛）处理10天、30天和100天，并将其腹侧前列腺与未处理的同龄对照动物进行比较。在所研究的上皮标志物中，前列腺特异性酸性磷酸酶存在于大鼠增生性前列腺中。免疫反应与病变严重程度和治疗持续时间有较好的相关性。前列腺特异性抗原在对照大鼠和处理大鼠中均显示出同等的免疫反应性。增生性和正常大鼠前列腺对其他上皮细胞标志物如上皮膜抗原、癌胚抗原和甲胎蛋白抗血清均未显示免疫反应性。得出结论，前列腺特异性酸性磷酸酶以及在较小程度上前列腺特异性抗原，可能是啮齿动物前列腺增生比较研究中有价值的标志物。
