单克隆抗体对末端补体复合物形成和细胞裂解的抑制作用。
Inhibition of terminal complement complex formation and cell lysis by monoclonal antibodies.
作者信息
Würzner R, Schulze M, Happe L, Franzke A, Bieber F A, Oppermann M, Götze O
机构信息
Department of Immunology, University of Göttingen, FRG.
出版信息
Complement Inflamm. 1991;8(5-6):328-40. doi: 10.1159/000463204.
Three monoclonal antibodies (mabs), two against C5 and one against C6, were identified and characterized. They inhibited the generation of the terminal complement complex (TCC) in serum to over 90% as assayed by a sensitive ELISA based on a neoepitope-specific mab, which recognized TCC-integrated C9. The haemolytic function of the TCC was markedly reduced by all three mabs implying that they are directed to epitopes on C5 and C6 which are essential for TCC formation in both the fluid phase and on erythrocyte membranes. Since the generation of C5a was also impaired by these mabs, they may serve as tools in investigations of the sequelae of the generation of C5a and of TCC.
鉴定并表征了三种单克隆抗体（mab），其中两种针对C5，一种针对C6。通过基于新表位特异性单克隆抗体的灵敏酶联免疫吸附测定（ELISA）检测，它们能将血清中末端补体复合物（TCC）的生成抑制至90%以上，该新表位特异性单克隆抗体可识别整合到TCC中的C9。所有这三种单克隆抗体均显著降低了TCC的溶血功能，这意味着它们作用于C5和C6上的表位，而这些表位对于液相和红细胞膜上TCC的形成至关重要。由于这些单克隆抗体也会损害C5a的生成，它们可作为研究C5a和TCC生成后遗症的工具。
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