Arévalo R, Alonso J R, Lara J, Briñón J G, Aijón J
Facultad de Biologia, Universidad de Salamanca.
J Hirnforsch. 1991;32(4):477-84.
The morphological characteristics of the synaptic contacts in the ruff of the cichlid fish Hemichromis bimaculatus were studies using the combined Golgi-electron microscope technique. Ruffed cells were located in the glomerular and plexiform layers and exhibited a pyriform or round cell body and numerous thin dendritic branches that were highly ramified. Four different segments could be distinguished on the initial portion of the axon (IP) according to the number and density of protrusions. These protrusions or lateral appendages are highly interdigitated, forming a characteristic synaptic field: the ruff. The ruff displayed a very high number of synapses with terminals showing a varied morphology. Protrusions of the ruff were both presynaptic and postsynaptic, taking also part in reciprocal pairs of synapses. Synapses from the ruff to the adjacent prolongation are asymmetrical, the prolongation to protrusion synapses being symmetrical. The axonal shaft participates in fewer synaptic contacts. Boutons contacting with one protrusion can synapt with other one, and can also receive an asymmetric synapse from another terminal, forming a serial synapse. This constitutes the most complex synaptic system observed in the glomerular layer of the olfactory bulb in any vertebrate. The synaptology of the ruffed cell IP is compared with previous reports on other species, with the teleostean mitral cells and with the IP of higher vertebrates neurons, the ruffed cells showing a completely different synaptic pattern.
运用高尔基染色法与电子显微镜技术相结合的方法，研究了双斑半丽鱼鳃耙中突触连接的形态特征。鳃耙细胞位于小球层和丛状层，呈梨形或圆形细胞体，并有许多高度分支的细树突分支。根据轴突起始段（IP）上突起的数量和密度，可区分出四个不同的节段。这些突起或侧支相互交错，形成一个特征性的突触场：鳃耙。鳃耙与形态各异的终末形成大量突触。鳃耙的突起既有突触前成分，也有突触后成分，还参与了突触对。从鳃耙到相邻延伸部分的突触是不对称的，从延伸部分到突起的突触是对称的。轴突干参与的突触联系较少。与一个突起接触的终扣可与另一个突起形成突触，也可从另一个终末接受不对称突触，形成串联突触。这构成了在任何脊椎动物嗅球小球层中观察到的最复杂的突触系统。将鳃耙细胞轴突起始段的突触学与之前关于其他物种、硬骨鱼二尖瓣细胞以及高等脊椎动物神经元轴突起始段的报道进行比较，鳃耙细胞呈现出完全不同的突触模式。
