Garcia-Verdugo J M, Llahi S, Farinas I, Martin V
J Hirnforsch. 1986;27(1):87-100.
In this study, the lamination pattern of the main olfactory bulb (MOB) of P. hispanica has been described using EM and Golgi-impregnation techniques. Six layers could be distinguished from the external surface to the ventricles: olfactory nerve layer (ONL), glomerular layer (GL), external plexiform layer (EPL), mitral layer (ML), internal plexiform layer (IPL), and granular cell layer (GCL). In ONL unmyelinated axonic bundles from the olfactory mucosa are seen. The GL is defined by the presence of terminal ramifications of the mitral primary dendrites and by axonic terminals of the olfactory nerve. The EPL is a dendritic articulation layer between the mitral and granule cells, where a great density of dendrodendritic synaptic contacts has been found. The ML is defined by the large mitral cell somata, and the IPL by myelinated axonic bundles that run rostro-caudally. Finally, the GCL is characterized by the presence of granule cells. Using the Golgi-impregnation method, five different neuronal types have been described. In the glomerular layer, a small cell population is located; these neurons seem the periglomerular cells described in mammals. The second type corresponds to the mitral cell population and the morphological features of these cells resemble to those of mammals. The three remaining types constitute the granule cell population; this population is characterized by a great morphological heterogeneousness. However, these types have been differentiated according to their dendritic tree morphology and location of the cell body. A morphological gradient depending on the distance of cell body for each type to the ependymal layer, has been observed. In EM, four different types of neuronal cell bodies according to their location and ultrastructure have been defined. The laminar organization of the MOB of Podarcis is similar to that in all vertebrates. The results for the fine structure and dendritic tree morphology have revealed a high similarity between these reptiles and mammals.
在本研究中，利用电子显微镜（EM）和高尔基染色技术描述了西班牙壁蜥主要嗅球（MOB）的分层模式。从外表面到脑室可区分出六层：嗅神经层（ONL）、肾小球层（GL）、外丛状层（EPL）、二尖瓣层（ML）、内丛状层（IPL）和颗粒细胞层（GCL）。在嗅神经层可见来自嗅黏膜的无髓轴突束。肾小球层由二尖瓣初级树突的终末分支和嗅神经的轴突终末的存在所定义。外丛状层是二尖瓣细胞和颗粒细胞之间的树突连接层，在该层发现了高密度的树突 - 树突突触联系。二尖瓣层由大的二尖瓣细胞体所定义，内丛状层由沿前后方向运行的有髓轴突束所定义。最后，颗粒细胞层的特征是存在颗粒细胞。使用高尔基染色法，已描述了五种不同的神经元类型。在肾小球层中，定位有一小群细胞；这些神经元似乎是哺乳动物中描述的球周细胞。第二种类型对应于二尖瓣细胞群，这些细胞的形态特征与哺乳动物的相似。其余三种类型构成颗粒细胞群；该群体的特征是具有很大的形态异质性。然而，这些类型已根据它们的树突形态和细胞体位置进行了区分。已观察到一种形态梯度，该梯度取决于每种类型的细胞体到室管膜层的距离。在电子显微镜下，根据其位置和超微结构定义了四种不同类型的神经元细胞体。西班牙壁蜥嗅球的分层组织与所有脊椎动物的相似。关于精细结构和树突形态的结果表明，这些爬行动物与哺乳动物之间具有高度相似性。
