Garbuglia A R, Manzin A, Budkowska A, Taliani G, Clementi M, Delfini C, Carloni G
Istituto Superiore di Sanità Rome, Italy.
J Med Virol. 1991 Dec;35(4):273-82. doi: 10.1002/jmv.1890350413.
The biological and clinical significances of pre-S antigens and HBV replication were investigated. Some 125 sera, 28 from HBeAg and 97 from anti-HBe-positive HBsAg, carriers were studied. The aim was to verify whether pre-S antigens could be expressed in serum in complete absence of viremia. Pre-S proteins, determined by an enzyme immunoassay, were found in sera regardless of the presence of HBV DNA, as detected by spot-hybridization. The sera without detectable HBV DNA were investigated further by PCR using specific primers for the S and C regions of HBV. PCR analysis of samples revealed that 4 out of 5 HBeAg and 33 out of 41 (80.5%) anti-HBe positive sera contained HBV-amplified sequences of S and C regions. Pre-S antigen values correlated well with the amounts of HBV DNA in serum detected by PCR in anti-HBe-positive subjects with high titers of pre-S antigens (10(4)-10(6)). In addition, PCR highlighted the presence of HBV DNA sequences in 8 out of 17 (47.1%) pre-S-negative HBsAg-positive sera.
对前S抗原和乙肝病毒复制的生物学及临床意义进行了研究。研究了约125份血清，其中28份来自HBeAg阳性携带者，97份来自抗-HBe阳性HBsAg携带者。目的是验证在前病毒血症完全不存在的情况下，前S抗原是否能在血清中表达。通过酶免疫测定法测定的前S蛋白在血清中被发现，而不论通过斑点杂交检测到的乙肝病毒DNA是否存在。对未检测到乙肝病毒DNA的血清，使用针对乙肝病毒S和C区域的特异性引物通过PCR进一步研究。样本的PCR分析显示，5份HBeAg阳性血清中有4份以及41份抗-HBe阳性血清中有33份（80.5%）含有乙肝病毒S和C区域的扩增序列。在前S抗原高滴度（10⁴ - 10⁶）的抗-HBe阳性受试者中，前S抗原值与通过PCR检测到的血清中乙肝病毒DNA量相关性良好。此外，PCR显示17份前S阴性HBsAg阳性血清中有8份（47.1%）存在乙肝病毒DNA序列。
