唐氏综合征患儿外周血细胞中的线粒体改变及凋亡倾向

Mitochondrial alterations and tendency to apoptosis in peripheral blood cells from children with Down syndrome.

作者信息

Roat Erika, Prada Nicole, Ferraresi Roberta, Giovenzana Chiara, Nasi Milena, Troiano Leonarda, Pinti Marcello, Nemes Elisa, Lugli Enrico, Biagioni Ornella, Mariotti Mauro, Ciacci Luigi, Consolo Ugo, Balli Fiorella, Cossarizza Andrea

机构信息

Chair of Immunology, Department of Biomedical Sciences, University of Modena and Reggio Emilia, via Campi 287, 41100 Modena, Italy.

出版信息

FEBS Lett. 2007 Feb 6;581(3):521-5. doi: 10.1016/j.febslet.2006.12.058. Epub 2007 Jan 17.

DOI:10.1016/j.febslet.2006.12.058

PMID:17250829

Abstract

Different types of cells from subjects with Down syndrome (DS) have an increased susceptibility to cell death. We have studied apoptosis and mitochondrial (mt) membrane potential (DeltaPsi(m)) in peripheral blood mononuclear cells (PBMC) from DS children and age-matched healthy donors after in vitro treatment with apoptogenic molecules, along with mtDNA content. We found that PBMC from DS and healthy controls had a similar tendency to undergo apoptosis and a similar amount of mtDNA. However, in cells from DS subjects, mitochondria showed a higher loss of DeltaPsi(m), underlying the presence of an increasing susceptibility of these organelles to damaging agents.

摘要

唐氏综合征（DS）患者的不同类型细胞对细胞死亡的易感性增加。我们研究了唐氏综合征患儿和年龄匹配的健康供体外周血单个核细胞（PBMC）在用凋亡分子进行体外处理后的凋亡情况和线粒体（mt）膜电位（ΔΨm），以及mtDNA含量。我们发现，唐氏综合征患者和健康对照者的PBMC发生凋亡的倾向相似，mtDNA含量也相似。然而，在唐氏综合征患者的细胞中，线粒体显示出更高的ΔΨm损失，这表明这些细胞器对损伤因子的易感性在增加。