Prímola-Gomes Thales N, Pires Washington, Rodrigues Luiz O C, Coimbra Cândido C, Marubayashi Umeko, Lima Nilo R V
Laboratory of Exercise Physiology, Department of Physical Education, School of Physical Education, Physical Therapy, and Occupational Therapy, Federal University of Minas Gerais, Av. Antônio Carlos, 6627, 31270-901 Belo Horizonte, Minas Gerais, Brazil.
Neurosci Lett. 2007 Feb 8;413(1):1-5. doi: 10.1016/j.neulet.2006.10.042. Epub 2007 Jan 23.
The aim of this study was to evaluate the effects of stimulation of the central cholinergic pathway on the regulation of post-exercise tail heat loss in rats. Either 2.0microL of 25x10(-3)M physostigmine (Phy) or 0.15M NaCl solution (Sal) were injected into the right lateral cerebral ventricle of both resting (n=8) and post-exercising rats (n=6; 24mmin(-1); 25min; 5% inclination). Tail temperature (Ttail) was measured using a thermistor taped to the tail, and intraperitoneal temperature, an index of core temperature (Tc), was recorded using a telemetry sensor implanted into the peritoneal cavity. In resting rats, Phy induced an increase in both Ttail (26.8+/-0.3 degrees C Phy versus 25.2+/-0.6 degrees C Sal; P<0.05) and in heat loss index (0.26+/-0.03 Phy versus 0.14+/-0.05 Sal; P<0.05; 30min after injection), and a decrease in Tc compared to the Sal injection group (36.6+/-0.2 degrees C Phy versus 37.0+/-0.2 degrees C Sal; P<0.05). In post-exercising rats, Phy injection attenuated the decrease in both T(tail) (28.3+/-0.8 degrees C Phy versus 26.4+/-0.6 degrees C Sal; P<0.05) and heat loss index (0.37+/-0.07 Phy versus 0.19+/-0.02 Sal; P<0.05) without altering Tc. We conclude that activation of the central cholinergic pathway increases post-exercise tail heat loss in rats.
本研究的目的是评估刺激中枢胆碱能通路对大鼠运动后尾部散热调节的影响。将2.0微升25×10⁻³M毒扁豆碱（Phy）或0.15M NaCl溶液（Sal）注入静息大鼠（n = 8）和运动后大鼠（n = 6；速度24米/分钟；持续25分钟；坡度5%）的右侧侧脑室。使用贴在尾巴上的热敏电阻测量尾温（Ttail），并使用植入腹腔的遥测传感器记录腹腔温度，作为核心温度（Tc）的指标。在静息大鼠中，Phy导致Ttail升高（Phy组为26.8±0.3℃，Sal组为25.2±0.6℃；P<0.05）以及散热指数升高（Phy组为0.26±0.03，Sal组为0.14±0.05；P<0.05；注射后30分钟），与注射Sal组相比，Tc降低（Phy组为36.6±0.2℃，Sal组为37.0±0.2℃；P<0.05）。在运动后大鼠中，注射Phy减弱了T(tail)的降低（Phy组为28.3±0.8℃，Sal组为26.4±0.6℃；P<0.05）和散热指数的降低（Phy组为0.37±0.07，Sal组为0.19±0.02；P<0.05），且未改变Tc。我们得出结论，中枢胆碱能通路的激活增加了大鼠运动后尾部的散热。
