Popik W, Inglot A D
Laboratory of Virology, Polish Academy of Sciences, Wrocław.
Arch Immunol Ther Exp (Warsz). 1991;39(1-2):19-26.
The effects of interferons (IFNs) and transforming growth factor-beta (TGF-beta) used alone and in combination on the multiplication of human embryonic diploid fibroblasts were studied. The experimental conditions were standardized and medium containing 2% of a single batch of fetal calf serum, which was almost minimal dose required for good the cell attachment and growth, was used. The observed effects were dose related. IFNs (types alpha, beta, or gamma) at concentration of 1 to 100 U/ml or TGF-beta at concentration of 0.1 to 1.0 ng/ml stimulated the cell multiplication by 20 to 25% when compared to the control cultures incubated without the factors. At higher doses both IFNs (10(2) to 10(5) U/ml) or TGF-beta (1 to 10 ng/ml) inhibited the proliferation of the cells. The antimitogenic action of IFNs and TGF-beta was synergistic. We suggest that IFNs as well as TGF-beta are bifunctional growth regulators although their antimitotic action dominates over their possible growth stimulatory activity. The latter action may be a secondary phenomenon due to interaction of IFNs or TGF-beta with other factors present in cell culture. We have shown that epidermal growth factor (EGF) may increase the growth stimulatory action of low doses of IFN-gamma or TGF-beta.
研究了单独使用以及联合使用干扰素（IFNs）和转化生长因子-β（TGF-β）对人胚二倍体成纤维细胞增殖的影响。实验条件已标准化，并使用了含有2%单一批次胎牛血清的培养基，这几乎是细胞良好附着和生长所需的最低剂量。观察到的效应与剂量相关。与未添加这些因子的对照培养物相比，浓度为1至100 U/ml的IFNs（α、β或γ型）或浓度为0.1至1.0 ng/ml的TGF-β可使细胞增殖提高20%至25%。在更高剂量下，IFNs（10²至10⁵ U/ml）或TGF-β（1至10 ng/ml）均会抑制细胞增殖。IFNs和TGF-β的抗有丝分裂作用具有协同性。我们认为IFNs以及TGF-β都是双功能生长调节剂，尽管它们的抗有丝分裂作用比其可能的生长刺激活性更为显著。后一种作用可能是由于IFNs或TGF-β与细胞培养中存在的其他因子相互作用而产生的次要现象。我们已经表明，表皮生长因子（EGF）可能会增强低剂量IFN-γ或TGF-β的生长刺激作用。
