一种用于单层培养物冷冻断裂的简单方法。
A simple method for freeze-fracture of monolayer cultures.
作者信息
Collins T R, Bartholomew J C, Calvin M
出版信息
J Cell Biol. 1975 Dec;67(3):904-11. doi: 10.1083/jcb.67.3.904.
Abstract
A simple method is described for the freeze-fracture in situ of monolayer cultures grown on gold carriers coated with a thin layer of silicon monoxide. Preliminary observations on 3T3 mouse embryo fibroblasts indicate that this technique exposes large areas of cell membrane, making it possible to determine how areas of membrane specialization are related to the cell as a whole and to regions of cellular interaction. 3T3 cells cultured on silicon monoxide show no modification of growth properties compared to cells growing on Falcon plastic, and other cell lines also appear to grow well on this substrate.
摘要
描述了一种用于在涂有一氧化硅薄层的金载体上生长的单层培养物原位冷冻断裂的简单方法。对3T3小鼠胚胎成纤维细胞的初步观察表明，该技术可暴露大面积的细胞膜，从而有可能确定膜特化区域与整个细胞以及细胞相互作用区域之间的关系。与在Falcon塑料上生长的细胞相比，在一氧化硅上培养的3T3细胞生长特性没有改变，并且其他细胞系在该基质上似乎也生长良好。
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