单层培养物的冷冻断裂
Freeze-fracture of monolayer cultures.
作者信息
Pauli B, Weinstein R S, Soble L W, Alroy J
出版信息
J Cell Biol. 1977 Mar;72(3):763-9. doi: 10.1083/jcb.72.3.763.
Abstract
This paper describes a simple method for the freeze-fracturing of cells in monolayers or multi-layer tissue cultures. The method produces high quality replicas and is applicable to the study of virtually any tissue culture or organ culture system. It uses standard materials and equipment for both tissue culture and freeze-fracturing.
摘要
本文描述了一种用于单层或多层组织培养中细胞冷冻断裂的简单方法。该方法可产生高质量的复制品，几乎适用于任何组织培养或器官培养系统的研究。它使用组织培养和冷冻断裂的标准材料和设备。
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Cultivation in vitro of cells derived from a human osteosarcoma.源自人骨肉瘤的细胞的体外培养。
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Structure of HeLa cells after treatment with glycerol as revealed by freeze-etching and electron microscopic methods.经冷冻蚀刻和电子显微镜方法揭示的甘油处理后海拉细胞的结构。
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Freeze-etching nomenclature.冷冻蚀刻命名法。
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