一种用于单层生长的细胞培养物冷冻断裂的改进技术。

An improved technique for the freeze-fracture of cell cultures grown as monolayers.

作者信息

Umrath W

出版信息

J Microsc. 1981 Feb;121(Pt 2):229-34. doi: 10.1111/j.1365-2818.1981.tb01217.x.

DOI:10.1111/j.1365-2818.1981.tb01217.x

Abstract

An improved and rapid procedure for the freeze-fracture of cell cultures grown as monolayers is described. No additional equipment than normally used for freeze-fracturing is necessary and cell culture conditions are as usual on plastic dishes. Cells are grown on a plastic foil membrane so that they can be viewed with a light microscope. If necessary, while viewing with a binocular microscope, specimen holders are glued to the foil membrane over selected areas of the culture. The specimen holders with the attached cell culture are dissected out and processed for freeze-fracturing in a BIOETH 2005. Fracture planes occur along the cell surface as well as through entire cells. Analysis of corresponding fracture faces is possible.

摘要

本文描述了一种用于单层生长的细胞培养物冷冻断裂的改进快速方法。除了通常用于冷冻断裂的设备外，无需额外设备，细胞培养条件与在塑料培养皿上的常规条件相同。细胞生长在塑料箔膜上，以便可以用光学显微镜观察。如有必要，在双目显微镜观察时，将标本固定器粘贴在培养物选定区域的箔膜上。将带有附着细胞培养物的标本固定器切下，并在BIOETH 2005中进行冷冻断裂处理。断裂平面沿着细胞表面以及穿过整个细胞出现。可以对相应的断裂面进行分析。