Szivek J A, Margolis D S, Schnepp A B, Grana W A, Williams S K
Orthopaedic Research Laboratory, Department of Orthopaedic Surgery, University of Arizona, Tucson, Arizona, USA.
J Biomed Mater Res A. 2007 Jun 15;81(4):939-47. doi: 10.1002/jbm.a.31116.
To develop implantable, engineered, cartilage constructs supported by a scaffold, techniques to encourage rapid tissue growth into, and on the scaffold are essential. Preliminary studies indicated that human endothelial cells proliferated at different rates on different calcium phosphate ceramic (CPC) particles. Judicious selection of particles may encourage specific cell proliferation, leading to an ordered growth of tissues for angiogenesis, osteogenesis, and chondrogenesis. The goal of this study was to identify CPC surfaces that encourage bone and vascular cell growth, and other surfaces that support chondrocyte growth while inhibiting proliferation of vascular cells. Differences in bone and vascular cell proliferation were observed when using epoxy without embedded CPCs to encourage bone cells, and when three CPCs were tested, which encouraged vascular cell proliferation. One of these (CPC 7) also substantially depressed cartilage cell proliferation. Only one small-diameter crystalline CPC (CPC 2) supported rapid chondrocyte proliferation, and maintained the cartilage cell phenotype.
为了开发由支架支撑的可植入工程化软骨构建体，促进组织快速长入支架并在支架上生长的技术至关重要。初步研究表明，人内皮细胞在不同的磷酸钙陶瓷（CPC）颗粒上以不同速率增殖。明智地选择颗粒可能会促进特定细胞增殖，从而导致组织有序生长以实现血管生成、骨生成和软骨生成。本研究的目的是确定促进骨细胞和血管细胞生长的CPC表面，以及支持软骨细胞生长同时抑制血管细胞增殖的其他表面。当使用不含嵌入CPC的环氧树脂来促进骨细胞生长时，以及测试三种促进血管细胞增殖的CPC时，观察到了骨细胞和血管细胞增殖的差异。其中一种（CPC 7）也显著抑制软骨细胞增殖。只有一种小直径结晶CPC（CPC 2）支持软骨细胞快速增殖，并维持软骨细胞表型。
