Szivek J A, Bliss C L, Geffre C P, Margolis D S, DeYoung D W, Ruth J T, Schnepp A B, Tellis B C, Vaidyanathan R K
Orthopaedic Research Laboratory, Department of Orthopaedic Surgery, University of Arizona, Tucson, AZ, USA.
J Biomed Mater Res B Appl Biomater. 2006 Nov;79(2):218-28. doi: 10.1002/jbm.b.30532.
No technique has been consistently successful in the repair of large focal defects in cartilage, particularly in older patients. Tissue-engineered cartilage grown on synthetic scaffolds with appropriate mechanical properties will provide an implant, which could be used to treat this problem. A means of monitoring loads and pressures acting on cartilage, at the defect site, will provide information needed to understand integration and survival of engineered tissues. It will also provide a means of evaluating rehabilitation protocols. A "sensate" scaffold with calibrated strain sensors attached to its surface, combined with a subminiature radio transmitter, was developed and utilized to measure loads and pressures during gait. In an animal study utilizing six dogs, peak loads of 120N and peak pressures of 11 MPa were measured during relaxed gait. Ingrowth into the scaffold characterized after 6 months in vivo indicated that it was well anchored and bone formation was continuing. Cartilage tissue formation was noted at the edges of the defect at the joint-scaffold interfaces. This suggested that native cartilage integration in future formulations of this scaffold configured with engineered cartilage will be a possibility.
在修复软骨的大面积局灶性缺损方面，尚无一种技术能始终取得成功，尤其是在老年患者中。在具有适当机械性能的合成支架上生长的组织工程软骨将提供一种植入物，可用于治疗这一问题。一种监测作用于缺损部位软骨的负荷和压力的方法，将提供了解工程组织整合和存活所需的信息。它还将提供一种评估康复方案的方法。开发了一种“传感”支架，其表面附着有校准应变传感器，并结合一个超小型无线电发射器，用于测量步态期间的负荷和压力。在一项利用6只狗的动物研究中，在轻松步态期间测量到的峰值负荷为120N，峰值压力为11MPa。体内6个月后对支架内生长情况的表征表明，它固定良好，骨形成仍在继续。在关节-支架界面处的缺损边缘发现了软骨组织形成。这表明，在这种配置有工程软骨的支架的未来配方中，天然软骨整合将是可能的。
