Lee Sira, Sim Sang-Jun, Park Chulhwan, Gu Man Bock, Hwang Un Yeon, Yi Jongheop, Oh Byung-Keun, Lee Jinwon
Department of Chemical and Biomolecular Engineering, Sogang University, Seoul 121-741, Korea.
J Microbiol Biotechnol. 2008 Oct;18(10):1695-700.
An immunosensor based on surface plasmon resonance (SPR) with enhanced performance was developed through a mixed self-assembled monolayer. A mixture of 16- mercaptohexadecanic acid (16-MHA) and 1-undecanethiol with various molar ratios was self-assembled on gold (Au) surface and the carboxylic acid groups of 16-MHA were then coordinated to Zn ions by exposing the substrate to an ethanolic solution of Zn(NO(3))(2)d6H2O. The antibody was immobilized on the SPR surface by exposing the functionalized substrate to the desired solution of antibody in phosphatebuffered saline (PBS) molecules. The film formation in series was confirmed by SPR and atomic force microscopy (AFM). The functionalized surface was applied to develop an SPR immunosensor for detecting human serum albumin (HSA) and the estimated detection limit (DL) was 4.27 nM. The limit value concentration can be well measured between ill and healthy conditions.
通过混合自组装单分子层开发了一种性能增强的基于表面等离子体共振（SPR）的免疫传感器。将具有不同摩尔比的16-巯基十六烷酸（16-MHA）和1-十一烷硫醇的混合物自组装在金（Au）表面，然后通过将底物暴露于Zn(NO₃)₂·6H₂O的乙醇溶液中，使16-MHA的羧酸基团与Zn离子配位。通过将功能化的底物暴露于磷酸盐缓冲盐水（PBS）分子中的所需抗体溶液，将抗体固定在SPR表面。通过SPR和原子力显微镜（AFM）确认了系列成膜情况。将功能化表面应用于开发用于检测人血清白蛋白（HSA）的SPR免疫传感器，估计检测限（DL）为4.27 nM。在患病和健康状态之间可以很好地测量极限值浓度。
