Gil Krzysztof, Słodowska-Hajduk Zofia, Bugajski Andrzej, Zieba-Nytko Ewa
Katedra Patofizjologii, Collegium Medicum Uniwersytetu Jagiellońskiego, Kraków.
Folia Med Cracov. 2005;46(3-4):23-42.
Exposure to inorganic dust containing silica particles leads to severe lung dysfunction. In order to evaluate the development of the early changes in pneumoconiosis the studies in rats were performed. Rats were instilled with silica and especially prepared coal dust by tracheal insuflation. After 1, 2, 3, 5, 8, and 10 weeks bronchial lavage was performed and the cells obtained were examined. The lung tissue samples were taken for histological evaluation. To assess the macrophages activity the nitro blue tetrazolium test (NBT-test) was performed. In the groups studied the differences in cell count and populations were found, especially in the monocytes and macrophages group of cells.
接触含有二氧化硅颗粒的无机粉尘会导致严重的肺功能障碍。为了评估尘肺病早期变化的发展情况，对大鼠进行了研究。通过气管内注入法给大鼠滴注二氧化硅和特别制备的煤尘。在1、2、3、5、8和10周后进行支气管灌洗，并对获得的细胞进行检查。采集肺组织样本进行组织学评估。为评估巨噬细胞活性，进行了硝基蓝四氮唑试验（NBT试验）。在所研究的组中发现了细胞计数和细胞群的差异，尤其是在单核细胞和巨噬细胞组。
