Höcker P, Wagner A
Intensivblutbank des Allgemeinen Krankenhauses, Stadt Wien.
Beitr Infusionsther. 1991;28:242-6.
Harvesting of peripheral blood stem cells (PBSC) by cytapheresis was performed in 57 patients, who underwent chemotherapy. The best yields were obtained when the leukocyte count was above 1 x 10(9)/l and the platelet count was raised above 80 x 10(9)/l. Using a Haemonetics V-50 or a Baxter CS-3000, 374 PBSC-aphereses were performed with a median of six aphereses per patient. The median number of PBSC (CFU-GM) retransfused in 22 patients who received PBSC for hematological reconstitution only was 3.26 x 10(4)/kg. For 22 patients who received autologous bone marrow plus BPSC, the median number of retransfused PBSC was 2.14 x 10(4)/kg. Myeloid engraftment was achieved in all patients, but megakaryopoiesis was delayed when the number of PBSC was less than 5.0 x 10(4)/kg. The results demonstrate that harvesting of a sufficient number of PBSC after chemotherapy is feasible but further measures like the use of rh GM-CSF will be necessary to reduce apheresis procedures and to obtain high yields to ensure rapid and complete engraftment.
对57例接受化疗的患者进行了血细胞分离术采集外周血干细胞（PBSC）。当白细胞计数高于1×10⁹/L且血小板计数升至高于80×10⁹/L时，收获量最佳。使用Haemonetics V - 50或Baxter CS - 3000进行了374次PBSC单采术，每位患者的单采术中位数为6次。仅接受PBSC进行血液重建的22例患者中，回输的PBSC（CFU - GM）中位数为3.26×10⁴/kg。对于22例接受自体骨髓加BPSC的患者，回输的PBSC中位数为2.14×10⁴/kg。所有患者均实现了髓系植入，但当PBSC数量少于5.0×10⁴/kg时，巨核细胞生成延迟。结果表明，化疗后采集足够数量的PBSC是可行的，但需要采取进一步措施，如使用重组人粒细胞巨噬细胞集落刺激因子（rh GM - CSF），以减少单采术次数并获得高产率，确保快速和完全植入。
