以硫代巴比妥酸反应物质和总还原型谷胱甘肽作为指标，对患有和未患有慢性阻塞性肺疾病的慢性吸烟者氧化应激的研究。

Study of thiobarbituric reactive substances and total reduced glutathione as indices of oxidative stress in chronic smokers with and without chronic obstructive pulmonary disease.

作者信息

Premanand R, Kumar Santhosh, Mohan Alladi

机构信息

Department of Tuberculosis and Chest Diseases, Division of Pulmonary and Critical Care Medicine, Sri Venkateswara Institute of Medical Sciences, Tirupati, Andhra Pradesh, India.

出版信息

Indian J Chest Dis Allied Sci. 2007 Jan-Mar;49(1):9-12.

PMID:17256560

Abstract

BACKGROUND

The imbalance between oxidants and antioxidants is thought to play an important role in the pathogenesis of chronic obstructive pulmonary disease (COPD).

METHODS

Biomarkers of lipid oxidation such as thiobarbituric reactive substances (TBARs), total reduced glutathione (GSH) were estimated in chronic smokers with and without COPD, and non-smokers.

RESULTS

The following subjects (all males) were studied: (i) chronic smokers (32.5 +/- 8.6 pack years) with stable COPD (n = 20; mean age 47.2 +/- 7.8 years); (ii) chronic smokers (6.3 +/- 1.9 pack years) without COPD (n = 20; mean age 35.3 +/- 4.5 years); and (iii) non-smokers (n = 20; mean age 37.5 +/- 5.5 years). The mean TBARs levels (nmol of malondialdehyde per ml of plasma) were higher among smokers with COPD (5.72 +/- 1.02) when compared with smokers without COPD (4.85 +/- 0.97) and non-smokers (2.58 +/- 0.56) (p < 0.001). The mean GSH levels (micromol/mg of protein) were significantly higher in non-smokers (0.163 +/- 0.113) compared to smokers with COPD (0.083 +/- 0.05) and those without COPD (0.050 +/- 0.051) (p < 0.001). There was no statistically significant difference in the plasma GSH levels among smokers with and without COPD (p > 0.05).

CONCLUSIONS

Our observations demonstrate increased lipid peroxidation because of oxidative stress due to smoking.

摘要

背景

氧化剂与抗氧化剂之间的失衡被认为在慢性阻塞性肺疾病（COPD）的发病机制中起重要作用。

方法

在患有和未患有COPD的慢性吸烟者以及非吸烟者中，评估脂质氧化的生物标志物，如硫代巴比妥酸反应性物质（TBARs）、总还原型谷胱甘肽（GSH）。

结果

研究了以下受试者（均为男性）：（i）患有稳定期COPD的慢性吸烟者（吸烟量32.5±8.6包年）（n = 20；平均年龄47.2±7.8岁）；（ii）未患有COPD的慢性吸烟者（吸烟量6.3±1.9包年）（n = 20；平均年龄35.3±4.5岁）；以及（iii）非吸烟者（n = 20；平均年龄37.5±5.5岁）。与未患有COPD的吸烟者（4.85±0.97）和非吸烟者（2.58±0.56）相比，患有COPD的吸烟者中TBARs的平均水平（每毫升血浆中丙二醛的纳摩尔数）更高（5.72±1.02）（p < 0.001）。与患有COPD的吸烟者（0.083±0.05）和未患有COPD的吸烟者（0.050±0.051）相比，非吸烟者中GSH的平均水平（微摩尔/毫克蛋白质）显著更高（0.163±0.113）（p < 0.001）。患有和未患有COPD的吸烟者血浆GSH水平之间无统计学显著差异（p > 0.05）。