Ambrus J L, Ambrus C M, Toumbis C A, Forgach P, Karakousis C P, Niswander P, Lane W
Roswell Park Cancer Institute, Buffalo, NY 14263.
J Med. 1991;22(6):355-69.
Methods were developed to test the angiogenic response to human tumor implants and various biologic agents in the cornea of rabbits and non-human primates (Macaca arctoides). Human malignant melanoma tissue and crude platelet derived growth factor (PDGF) preparations had significant angiogenic effects. Purified, recombinant PDGF preparations were also effective initiators. Hemorheologic agents which also inhibit platelet aggregation [e.g. pentoxifylline (Px) (Trental) (also found to release PgI2 and tissue plasminogen activator (t-PA)] inhibited human tumor implant-induced angiogenesis. Sodium diethyldithiocarbamate (DDTC), a metal complexing agent with special affinity to copper and anti-thyroid as well as immune stimulating activity, was shown to be anti-angiogenic and to increase the effect of Px. The anti-fibrinolytic agents epsilon amino caproic acid (EACA) and tranexamic acid (t-AMCHA) were anti-angiogenic.
已开发出多种方法来测试兔和非人灵长类动物（熊猴）角膜对人肿瘤植入物及各种生物制剂的血管生成反应。人恶性黑色素瘤组织和粗制血小板衍生生长因子（PDGF）制剂具有显著的血管生成作用。纯化的重组PDGF制剂也是有效的启动剂。同样抑制血小板聚集的血液流变学药物[如己酮可可碱（Px）（曲克芦丁）（也发现其可释放前列环素（PgI2）和组织纤溶酶原激活剂（t-PA）]可抑制人肿瘤植入物诱导的血管生成。二乙基二硫代氨基甲酸钠（DDTC）是一种对铜具有特殊亲和力、具有抗甲状腺及免疫刺激活性的金属络合剂，已证明其具有抗血管生成作用，并可增强己酮可可碱的效果。抗纤溶药物ε-氨基己酸（EACA）和氨甲环酸（t-AMCHA）具有抗血管生成作用。
