Kinoshita Takashi, Hoshino Tomoaki, Imaoka Haruki, Ichiki Hiroko, Okamoto Masaki, Kawayama Tomotaka, Yodoi Junji, Kato Seiya, Aizawa Hisamichi
Division of Respirology, Neurology, and Rheumatology, Department of Medicine, Kurume University School of Medicine, Kurume, Fukuoka 830-0011, Japan.
Biochem Biophys Res Commun. 2007 Mar 16;354(3):712-9. doi: 10.1016/j.bbrc.2007.01.053. Epub 2007 Jan 19.
Thioredoxin 1 (TRX1) is a redox (reduction/oxidation)-active protein that scavenges reactive oxygen species. Here we examined whether endogenous or exogenous administration of TRX1 prevented the development and progression of elastase-induced pulmonary emphysema. Mice were treated with intratracheal elastase via microspray on day 0, and were given recombinant human TRX1 (rhTRX1) every other day from days -1 to 21. To determine the effects of TRX1 on the progression of established emphysema, mice were treated intratracheally with elastase on day 0, and rhTRX1 was administered from days 14 to 21. Histopathologic examination was performed on day 21. TRX1-transgenic but not transgene-negative mice demonstrated a decrease in the physiological indicators of elastase-induced emphysema. TRX1 administration from days -1 to 19 significantly decreased the signs of elastase-induced emphysema. Moreover, TRX1 administration beginning 14 days after elastase treatment significantly slowed the progression of emphysema. TRX1 may be of clinical benefit for the treatment of COPD.
硫氧还蛋白1（TRX1）是一种具有氧化还原（还原/氧化）活性的蛋白质，可清除活性氧。在此，我们研究了内源性或外源性给予TRX1是否能预防弹性蛋白酶诱导的肺气肿的发生和发展。在第0天通过微量喷雾经气管给予小鼠弹性蛋白酶，并在第-1天至第21天每隔一天给予重组人TRX1（rhTRX1）。为了确定TRX1对已建立的肺气肿进展的影响，在第0天经气管给予小鼠弹性蛋白酶，并在第14天至第21天给予rhTRX1。在第21天进行组织病理学检查。TRX1转基因小鼠而非转基因阴性小鼠显示弹性蛋白酶诱导的肺气肿的生理指标有所下降。在第-1天至第19天给予TRX1可显著减轻弹性蛋白酶诱导性肺气肿的体征。此外，在弹性蛋白酶治疗14天后开始给予TRX1可显著减缓肺气肿的进展。TRX1可能对慢性阻塞性肺疾病的治疗具有临床益处。
