小鼠精子中磷酸肌醇依赖性途径受卵子ZP3调控并驱动顶体反应。
Phosphoinositide-dependent pathways in mouse sperm are regulated by egg ZP3 and drive the acrosome reaction.
作者信息
Jungnickel Melissa K, Sutton Keith A, Wang Yanli, Florman Harvey M
机构信息
Department of Cell Biology, University of Massachusetts, 55 Lake Avenue North, Worcester, MA 01655, USA.
出版信息
Dev Biol. 2007 Apr 1;304(1):116-26. doi: 10.1016/j.ydbio.2006.12.023. Epub 2006 Dec 15.
Abstract
Sperm of many animals must complete an exocytotic event, the acrosome reaction, in order to fuse with eggs. In mammals, acrosome reactions are triggered during sperm contact with the egg extracellular matrix, or zona pellucida, by the matrix glycoprotein ZP3. Here, we show that ZP3 stimulates production of phosphatidylinositol-(3,4,5)-triphosphate in sperm membranes. Phosphatidylinositol-3-kinase antagonists that prevent acrosome reactions and fertilization in vitro, while generation of this phosphoinositide in the absence of ZP3 triggered acrosome reactions. Downstream effectors of phosphatidylinositol-(3,4,5)-triphosphate in sperm include the protein kinases, Akt and PKCzeta. These studies outline a signal transduction pathway that plays an essential role in the early events of mammalian fertilization.
摘要
许多动物的精子必须完成一种胞吐事件，即顶体反应，才能与卵子融合。在哺乳动物中，精子与卵子的细胞外基质（即透明带）接触时，基质糖蛋白ZP3会触发顶体反应。在此，我们表明ZP3刺激精子膜中磷脂酰肌醇-（3,4,5）-三磷酸的产生。在体外阻止顶体反应和受精的磷脂酰肌醇-3-激酶拮抗剂，而在没有ZP3的情况下这种磷酸肌醇的产生会触发顶体反应。精子中磷脂酰肌醇-（3,4,5）-三磷酸的下游效应器包括蛋白激酶Akt和PKCζ。这些研究概述了一条在哺乳动物受精早期事件中起关键作用的信号转导途径。
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