Britos Leticia, Lalanne Ana Inés, Castillo Estela, Cota Germán, Señorale Mario, Marín Mónica
Sección Bioquímica, Facultad de Ciencias, Universidad de la República, Iguá 4225, CP 11400, Montevideo, Uruguay.
Exp Parasitol. 2007 Jun;116(2):95-102. doi: 10.1016/j.exppara.2006.11.008. Epub 2007 Jan 26.
With the aim of identifying genes involved in development and parasite adaptation in cestodes, four coding sequences were isolated from the cyclophyllidean Mesocestoides corti larval stage (tetrathyridium). Genes showed significant similarity to the cysteine-rich secreted protein (CRISP) encoding genes, a large family that includes stage and tissue-specific genes from diverse organisms, many associated with crucial biological processes. The full-length McCrisp2 cDNA encodes a predicted protein of 202 residues in length, containing 10 cysteines and a putative signal peptide. The expression level of McCrisp2 was estimated by Real-time PCR, relative to GAPDH, showing an increase of 75% in segmented worms compared to tetrathyridia. By in situ hybridization, McCrisp2 expression was localized mainly at the larvae apical region of tetrathyridia and in the proglottids of segmented worms. Taken together our results suggest a possible role for M. corti CRISP proteins as ES products, potentially involved in differentiation processes as proposed for homologs in other organisms.
为了鉴定与绦虫发育和寄生虫适应性相关的基因，从圆叶目绦虫中绦期幼虫（四膜虫）分离出四个编码序列。这些基因与富含半胱氨酸的分泌蛋白（CRISP）编码基因具有显著相似性，CRISP是一个大家族，包含来自不同生物体的阶段和组织特异性基因，许多基因与关键生物学过程相关。全长的McCrisp2 cDNA编码一个预测长度为202个氨基酸的蛋白质，含有10个半胱氨酸和一个假定的信号肽。通过实时PCR相对于GAPDH估计McCrisp2的表达水平，结果显示，与四膜虫相比，成虫节片中McCrisp2的表达增加了75%。通过原位杂交，McCrisp2的表达主要定位于四膜虫幼虫的顶端区域和成虫节片中。综合我们的结果表明，中绦虫CRISP蛋白可能作为排泄分泌产物发挥作用，可能如其他生物体中的同源物一样参与分化过程。
