Costa Caroline B, Monteiro Karina M, Teichmann Aline, da Silva Edileuza D, Lorenzatto Karina R, Cancela Martín, Paes Jéssica A, Benitz André de N D, Castillo Estela, Margis Rogério, Zaha Arnaldo, Ferreira Henrique B
Laboratório de Genômica Estrutural e Funcional,Laboratório de Biologia Molecular de Cestódeos, andPrograma de Pós-Graduação em Biologia Celular e Molecular (PPGBCM), Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS),Porto Alegre,RS,Brazil.
Sección Bioquímica y Biología Molecular,Facultad de Ciencias,Universidad de la República,Montevideo,Uruguay.
Parasitology. 2015 Aug;142(9):1171-82. doi: 10.1017/S003118201500030X. Epub 2015 Mar 31.
The histone chaperone SET/TAF-Iβ is implicated in processes of chromatin remodelling and gene expression regulation. It has been associated with the control of developmental processes, but little is known about its function in helminth parasites. In Mesocestoides corti, a partial cDNA sequence related to SET/TAF-Iβ was isolated in a screening for genes differentially expressed in larvae (tetrathyridia) and adult worms. Here, the full-length coding sequence of the M. corti SET/TAF-Iβ gene was analysed and the encoded protein (McSET/TAF) was compared with orthologous sequences, showing that McSET/TAF can be regarded as a SET/TAF-Iβ family member, with a typical nucleosome-assembly protein (NAP) domain and an acidic tail. The expression patterns of the McSET/TAF gene and protein were investigated during the strobilation process by RT-qPCR, using a set of five reference genes, and by immunoblot and immunofluorescence, using monospecific polyclonal antibodies. A gradual increase in McSET/TAF transcripts and McSET/TAF protein was observed upon development induction by trypsin, demonstrating McSET/TAF differential expression during strobilation. These results provided the first evidence for the involvement of a protein from the NAP family of epigenetic effectors in the regulation of cestode development.
组蛋白伴侣SET/TAF-Iβ参与染色质重塑和基因表达调控过程。它与发育过程的控制有关，但对其在蠕虫寄生虫中的功能了解甚少。在中殖孔绦虫中，在筛选幼虫（四膜虫）和成虫中差异表达的基因时，分离出了一段与SET/TAF-Iβ相关的部分cDNA序列。在此，对中殖孔绦虫SET/TAF-Iβ基因的全长编码序列进行了分析，并将编码的蛋白质（McSET/TAF）与直系同源序列进行了比较，结果表明McSET/TAF可被视为SET/TAF-Iβ家族成员，具有典型的核小体组装蛋白（NAP）结构域和酸性尾巴。通过RT-qPCR（使用一组五个参考基因）以及免疫印迹和免疫荧光（使用单特异性多克隆抗体），研究了McSET/TAF基因和蛋白质在绦虫节片形成过程中的表达模式。在用胰蛋白酶诱导发育后，观察到McSET/TAF转录本和McSET/TAF蛋白逐渐增加，这表明在节片形成过程中McSET/TAF存在差异表达。这些结果首次证明了表观遗传效应器NAP家族中的一种蛋白质参与了绦虫发育的调控。
