Andonov M D, Chaldakov G N
Department of Anatomy and Histology, Varna Institute of Medicine, Bulgaria.
Acta Histochem Suppl. 1991;41:55-63.
On the ultrastructural level, the role of Ca2+ and cAMP in the rat spermatogenesis was studied. Using a K-pyroantimonate method for intracellular localization of Ca2+, precipitates were found within (i) the vesicular component of the chromatoid body, (ii) vesicular elements of the trans-Golgi area including coated vesicles, (iii) smooth endoplasmic reticulum vesicles and cisterns accompanying the perinuclear (manchette) microtubules, and (iv) mid-piece mitochondria of germ and Sertoli cells. The results suggest a close relationship between the Ca(2+)-containing smooth endoplasmic reticulum and the control of microtubule assembly within microdomains. Theophylline (a cAMP-phosphodiesterase inhibitor) treatment of rats (96 mg/kg daily i.p. over 5 days) caused a significant increase of (a) the GERL-related coated vesicles and (b) the number of manchette microtubules.
在超微结构水平上，研究了Ca2+和cAMP在大鼠精子发生中的作用。采用焦锑酸钾法对细胞内Ca2+进行定位，在以下部位发现了沉淀物：(i) 拟染色体体的囊泡成分；(ii) 反式高尔基体区域的囊泡结构，包括被膜小泡；(iii) 伴随核周（袖套）微管的滑面内质网小泡和池；(iv) 生殖细胞和支持细胞的中段线粒体。结果表明，含Ca(2+)的滑面内质网与微区内微管组装的控制之间存在密切关系。用茶碱（一种cAMP磷酸二酯酶抑制剂）处理大鼠（每天腹腔注射96 mg/kg，持续5天），导致(a) 与GERL相关的被膜小泡和(b) 袖套微管数量显著增加。
