Proulx Alain A, Gonder John R
Ivey Eye Institute, London, Ontario, Canada.
Am J Ophthalmol. 2007 Feb;143(2):353-4. doi: 10.1016/j.ajo.2006.09.035. Epub 2006 Oct 17.
To compare the in vitro effect of a single brief indocyanine green (ICG) exposure with a double exposure on retinal pigment epithelial (RPE) cells.
In vitro laboratory experimental study.
Human ARPE-19 cells were exposed to a single dilute ICG exposure (0.5 mg/ml) or to two sequential exposures of identical volume and concentration. Viability was measured with a mitochondrial dehydrogenase assay and compared with nonexposed control cells.
Cell viability was not statistically different between the single-exposed, double-exposed, or control cell populations.
When used intraoperatively to stain the internal limiting membrane, dilute ICG dye sometimes does not adequately enhance visualization of the internal limiting membrane. Occasionally, it is necessary to repeat the dye exposure to achieve the desired visibility. In this study, RPE cells subjected to two consecutive short exposures of ICG showed no marked difference in cell survival when compared with cells exposed to a single application of ICG and to control cells.
比较单次短暂吲哚菁绿（ICG）暴露与两次暴露对视网膜色素上皮（RPE）细胞的体外作用。
体外实验室实验研究。
将人ARPE - 19细胞暴露于单次稀释的ICG暴露（0.5 mg/ml）或两次相同体积和浓度的连续暴露。用线粒体脱氢酶测定法测量细胞活力，并与未暴露的对照细胞进行比较。
单次暴露、两次暴露或对照细胞群体之间的细胞活力无统计学差异。
术中使用稀释的ICG染料染色内界膜时，有时不能充分增强内界膜的可视化。偶尔，有必要重复染料暴露以达到所需的可见度。在本研究中，与单次应用ICG的细胞和对照细胞相比，接受两次连续短时间ICG暴露的RPE细胞在细胞存活率方面无明显差异。
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