Ikagawa Hiroshi, Yoneda Masahiko, Iwaki Masayoshi, Isogai Zenzo, Tsujii Kaoru, Yamazaki Ritsuko, Kamiya Tetsurou, Zako Masahiro
Department of Ophthalmology, Aichi Medical University, Nagakute, Aichi, Japan.
Invest Ophthalmol Vis Sci. 2005 Jul;46(7):2531-9. doi: 10.1167/iovs.04-1521.
To investigate the chemical toxicity of indocyanine green (ICG).
Surface active and precipitating effects of ICG were quantitatively analyzed by determining bovine serum albumin dissolved or precipitated in the presence or absence of salt solutions. The effects of precipitation on serum and cytotoxicity were evaluated by measuring the viability of retinal pigment epithelium (RPE) in vitro.
ICG functioned as a surfactant without salts, but with nearly physiological concentrations of balanced salts, it functioned as a unique precipitating factor. This rendered the soluble molecules in serum that are indispensable in the culture of RPE cells insoluble during a 12-hour exposure, resulting in poor cell survival in vitro. Cytotoxicity in serum-free medium was also shown during brief exposures.
Commonly used dosages of ICG directly applied into the vitreous cavity, which not only contact the retina but also invade the space between the retina and RPE through a macular hole, may be sufficient to induce retinal disorders after the damaging chemical property of ICG has disturbed the microenvironment.
研究吲哚菁绿（ICG）的化学毒性。
通过测定在有或无盐溶液存在的情况下溶解或沉淀的牛血清白蛋白，定量分析ICG的表面活性和沉淀作用。通过测量体外视网膜色素上皮（RPE）的活力，评估沉淀对血清和细胞毒性的影响。
在无盐情况下，ICG起表面活性剂的作用，但在接近生理浓度的平衡盐存在时，它起独特的沉淀因子的作用。这使得RPE细胞培养中不可或缺的血清中的可溶性分子在12小时暴露期间变得不溶，导致体外细胞存活率低。在短暂暴露期间，无血清培养基中也显示出细胞毒性。
直接注入玻璃体腔的常用剂量的ICG，不仅接触视网膜，还会通过黄斑裂孔侵入视网膜和RPE之间的间隙，在ICG的有害化学性质扰乱微环境后，可能足以诱发视网膜疾病。
