Mamiya Takayoshi, Kise Mitsuo, Morikawa Keiko, Aoto Hiromichi, Ukai Makoto, Noda Yukihiro
Department of Chemical Pharmacology, Faculty of Pharmacy, Meijo University, 150 Yagotoyama, Tempaku-ku, Nagoya 468-8503, Japan.
Pharmacol Biochem Behav. 2007 Jan;86(1):62-7. doi: 10.1016/j.pbb.2006.12.008. Epub 2006 Dec 22.
We investigated the antidepressant-like effects of pre-germinated brown rice (PGBR) and polished rice (PR) pellets, respectively, in comparison with control (AIN-93G) pellets in the forced swimming test and the learned helplessness paradigm in mice. Mice were fed respective pellets for 30 days. The immobility time on the 2nd day of the forced swimming test was shorter in mice fed with PR or PGBR pellets than in mice fed with control pellets. In the learned helplessness paradigm, the number of escape failures in mice fed with PGBR pellets was significantly smaller than that in mice fed with control pellets. Compared to the control group, an increase in serotonin (5-HT) levels, but not in 5-hydroxyindoleacetic acid (5-HIAA) levels, and a decrease in the 5-HIAA/5-HT ratio were observed in the frontal cortex of the PGBR group. There were no differences among the three groups in terms of 5-HT and 5-HIAA levels and their ratios in the hippocampus and striatum. The levels of noradrenaline and 3-methoxy-4-hydroxyphenylglycol were not affected by the food pellets in all the brain regions tested. Additionally, we could not detect any differences in the expression of the 5-HT1A receptor and the 5-HT transporter in the frontal cortex of the three groups. These results suggest that the increase of 5-HT levels in the mouse frontal cortex contributes to the antidepressant-like effects of PGBR pellets.
我们分别研究了预发芽糙米（PGBR）和精米（PR）颗粒在强迫游泳试验和小鼠习得性无助范式中的抗抑郁样作用，并与对照（AIN-93G）颗粒进行比较。小鼠分别喂食相应颗粒30天。在强迫游泳试验的第2天，喂食PR或PGBR颗粒的小鼠的不动时间比喂食对照颗粒的小鼠短。在习得性无助范式中，喂食PGBR颗粒的小鼠的逃避失败次数显著少于喂食对照颗粒的小鼠。与对照组相比，PGBR组额叶皮质中血清素（5-HT）水平升高，但5-羟吲哚乙酸（5-HIAA）水平未升高，5-HIAA/5-HT比值降低。三组在海马体和纹状体中的5-HT和5-HIAA水平及其比值方面没有差异。在所测试的所有脑区中，去甲肾上腺素和3-甲氧基-4-羟基苯乙二醇的水平均不受食物颗粒的影响。此外，我们未检测到三组额叶皮质中5-HT1A受体和5-HT转运体表达的任何差异。这些结果表明，小鼠额叶皮质中5-HT水平的升高有助于PGBR颗粒的抗抑郁样作用。
