Yang Ling, Chen Zhi-liang, Liu Shi-ting, Yang Li
Department of Pharmacy, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2006 Dec;26(12):1706-8.
To develop a method for quantitative collection of exhaled gas in anesthetized dogs at given time following intravenous administration of octafluoropropane (OFP)-containing human albumin micropheres for assessing the gas kinetics of OFP.
OFP-containing albumin micropheres were administered intravenously at 0.4, 0.8 and 1.2 ml/kg, respectively, in anesthetized and ventilated dogs. The exhaled air samples were analyzed by gas chromatography-tandem mass spectrometry (GC-MS-MS).
The correlation curve between the area under curve (AUC) and administered dose was roughly linear (Y=1162.5X-417.38, r square=0.949 9). The total recovery rate was (119.49-/+27.62)% which was not significantly different from the rate of 100% (P>0.05). GC-MS-MS was accurate, sensitive, precise and applicable for OFP determination.
The sampling method is useful for characterizing OFP pharmacokinetics in dogs, and also applicable for studying the pharmacokinetics of other gas-containing drugs.
建立一种在静脉注射含八氟丙烷（OFP）的人白蛋白微球后，于给定时间对麻醉犬呼出气体进行定量收集的方法，以评估OFP的气体动力学。
分别以0.4、0.8和1.2 ml/kg的剂量对麻醉且通气的犬静脉注射含OFP的白蛋白微球。呼出气体样本通过气相色谱-串联质谱（GC-MS-MS）进行分析。
曲线下面积（AUC）与给药剂量之间的相关曲线大致呈线性（Y = 1162.5X - 417.38，决定系数r² = 0.949 9）。总回收率为（119.49 ± 27.62）%，与100%的回收率无显著差异（P > 0.05）。GC-MS-MS准确、灵敏、精密，适用于OFP的测定。
该采样方法有助于表征犬体内OFP的药代动力学，也适用于研究其他含气体药物的药代动力学。
