[胎鼠肝细胞中巢蛋白阳性细胞的分离、培养及诱导分化]

[Isolation, culture, and differentiation induction of nestin-positive cells in fetal rat hepatic cells].

作者信息

Liu Jun, An Jing, Cehn Jin-quan

机构信息

Department of Histology and Embryology, Southern Medical University, Guangzhou 510515, China.

出版信息

Nan Fang Yi Ke Da Xue Xue Bao. 2006 Dec;26(12):1757-9.

PMID:17259114

Abstract

OBJECTIVE

To establish the method for isolation, culture, and differentiation induction of nestin-positive cells in fetal rat hepatic cells.

METHODS

Hepatic cells were obtained from fetal rats by means of mechanical separation and hanging-drop culture, and after two days of primary culture, the medium was changed for further cell culture in the presence of 20% fetal bovine serum (containing glucose 25 ml/L, mycillin 100 U/ml, pH 7.6), 10 mmol/L nicotinamide, 1 mg/L insulin, affix N2, basic fibroblast growth factor, stem cell factor, epidermal growth factor and leukemia inhibitory factor.

RESULT AND CONCLUSION

Nestin-positive cells were obtained from fetal rat liver, which can differentiate into islet beta cells after culture and expansion in vitro.

摘要

目的

建立胎鼠肝细胞中巢蛋白阳性细胞的分离、培养及诱导分化方法。

方法

采用机械分离和悬滴培养法从胎鼠获取肝细胞，原代培养两天后，更换培养基，在含有20%胎牛血清（含葡萄糖25 ml/L、青霉素100 U/ml、pH 7.6）、10 mmol/L烟酰胺、1 mg/L胰岛素、添加N2、碱性成纤维细胞生长因子、干细胞因子、表皮生长因子和白血病抑制因子的条件下进行进一步细胞培养。