Ratomski Karol, Wysocka Jolanta, Skotnicka Bozena, Kasprzycka Edwina, Zelazowska-Rutkowska Beata, Zak Janusz, Hassmann-Poznańska Elzbieta
Zakład Laboratoryjnej Diagnostyki Pediatrycznej, Uniwersytet Medyczny w Białymstoku.
Otolaryngol Pol. 2009 Mar-Apr;63(2):131-5. doi: 10.1016/S0030-6657(09)70093-9.
Chemokine receptors play a crucial role in the recruitment of leucocytes into inflamed tissue from secondary lymphoid organs.
was evaluation of the percentage Th (CD4+) lymphocytes with expression of chemokine receptors: CCR3, CCR4, and CCR5 in hypertrophied adenois tissue in children with otitis media with effusion.
36 children with otitis media with effusion and 25 children with hypertrophied adenoids were tested. Expression of chemokine receptors CCR3, CCR4 and CCR5 on T CD4 lymphocytes of hypertrophied adenoid's tissue was estimated by flow cytometry method.
Average percentage of T CD4+ lymphocytes with expression CCR4 in hypertrophied adenoid in children group with otitis media with effusion (OME = 64.11%) was significantly higher than in comparative group with hypertrophied adenoid (HA = 75.05%, p < 0.04). We showed statistically lower percentage of CD3+CD4+ cells with expression CCR3 in examinated group (OME = 47.19%) than HA group (62.66%; p < 0.003).
Results suggests that intensify of humoral immunological answer in hypertrophied adenoid depends on Th2 lymphocytes with expression CCR3 and CCR4 chemokine receptors in course of inflammatory state in middle ear.
趋化因子受体在将白细胞从二级淋巴器官募集到炎症组织中起着关键作用。
评估患有渗出性中耳炎的儿童肥大腺样体组织中表达趋化因子受体CCR3、CCR4和CCR5的Th（CD4+）淋巴细胞百分比。
对36例渗出性中耳炎患儿和25例腺样体肥大患儿进行检测。采用流式细胞术方法评估肥大腺样体组织中T CD4淋巴细胞上趋化因子受体CCR3、CCR4和CCR5的表达。
患有渗出性中耳炎的儿童组（OME = 64.11%）肥大腺样体中表达CCR4的T CD4+淋巴细胞平均百分比显著高于腺样体肥大对照组（HA = 75.05%，p < 0.04）。我们发现，检测组中表达CCR3的CD3+CD4+细胞百分比（OME = 47.19%）在统计学上低于HA组（62.66%；p < 0.003）。
结果表明，在中耳炎症状态过程中，肥大腺样体中体液免疫应答的增强取决于表达CCR3和CCR4趋化因子受体的Th2淋巴细胞。
