[中耳积液患儿肥大腺样体中B淋巴细胞上CD80和CD86共受体的表达]
[CD80 and CD86 coreceptors expression on lymphocytes B in hypertrophied adenoids in children with otitis media with effusion].
作者信息
Ratomski Karol, Zelazowska-Rutkowska Beata, Wysocka Jolanta, Skotnicka Bozena, Kasprzycka Edwina, Ilona Bauer
机构信息
Zaklad Laboratoryjnej Diagnostyki, Pediatrycznej Uniwersytetu Medycznego w Białymstoku.
出版信息
Otolaryngol Pol. 2010 Mar-Apr;64(2):93-7. doi: 10.1016/S0030-6657(10)70042-1.
AIM
The aim of this study was evaluation of the percentage and mean fluorescence intensity of lymphocytes B with expression CD80 and CD86 in hypertrophied adenoids in children with otitis media with effusion.
MATERIAL AND METHODS
Expression coreceptors CD80 and CD86 on lymphocytes B in adenoid tissue were estimated by flow cytometry method. 15 children with otitis media with effusion and 15 children with hypertrophied adenoid were tested.
RESULTS
This study showed significantly higher percentage of lymphocytes CD19+ CD80+ in children with otitis media with effusion (OME 1.42% +/- 0.91) than in comparative group with hypertrophied adenoids (AH 0.63% +/- 0.21). The receptor CD86 didn't show differences in expression on lymphocytes B between both examinated groups.
CONCLUSION
Low expression of CD80 and CD86 indicates that local immunity response in hypertrophied adenoids in children with otitis media with effusion is weakened. It can contribute to the otitis media with effusion.
目的
本研究旨在评估患有渗出性中耳炎的儿童肥大腺样体中表达CD80和CD86的B淋巴细胞的百分比及平均荧光强度。
材料与方法
采用流式细胞术检测腺样体组织中B淋巴细胞上共刺激分子CD80和CD86的表达。对15例渗出性中耳炎患儿和15例腺样体肥大患儿进行了检测。
结果
本研究显示，渗出性中耳炎患儿（OME，1.42%±0.91）中CD19+CD80+淋巴细胞的百分比显著高于腺样体肥大对照组（AH，0.63%±0.21）。在两组检测的B淋巴细胞上，共刺激分子CD86的表达未显示出差异。
结论
CD80和CD86的低表达表明，患有渗出性中耳炎的儿童肥大腺样体中的局部免疫反应减弱。这可能导致渗出性中耳炎。
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