微小隐孢子虫的子孢子和裂殖子共享一个由单克隆抗体和二维电泳识别的共同表位。
Sporozoites and merozoites of Cryptosporidium parvum share a common epitope recognized by a monoclonal antibody and two-dimensional electrophoresis.
作者信息
Tilley M, Upton S J
机构信息
Division of Biology, Kansas State University, Manhattan 66506.
出版信息
J Protozool. 1991 Nov-Dec;38(6):48S-49S.
PMID:1726328
Abstract
Sporozoites and merozoites of Cryptosporidium parvum were analyzed for the presence of a 15 kDa surface antigen using a monoclonal antibody probe. Both were found to possess the antigen by immunofluorescence, and further analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis confirmed these observations. When separated by two-dimensional electrophoresis the isoelectric point was found to be similar, with major spots at 4.25 and minor spots at 4.15.
摘要
使用单克隆抗体探针分析微小隐孢子虫的子孢子和裂殖子是否存在15 kDa表面抗原。通过免疫荧光发现两者均含有该抗原，并且通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳进行的进一步分析证实了这些观察结果。当通过二维电泳分离时，发现等电点相似，主要斑点在4.25，次要斑点在4.15。
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