Kaneshiro E S, Wu Y P, Cushion M T
Department of Biological Sciences, University of Cincinnati, OH 45221-0006.
J Protozool. 1991 Nov-Dec;38(6):85S-87S.
A series of classical vital stains and fluorescent indicator compounds were evaluated as viability assays of P. carinii. The combination of the acetoxymethyl ester of calcein with either ethidium homodimer or propidium iodide distinguished between live, dead and moribund organisms and provided high fluorescence intensity and low bleaching enabling photodocumentation at high magnifications.
对一系列经典的活体染色剂和荧光指示剂化合物进行了评估，以作为卡氏肺孢子虫的生存力检测方法。钙黄绿素乙酰氧基甲酯与乙锭同二聚体或碘化丙啶的组合能够区分活的、死亡的和濒死的生物体，并提供高荧光强度和低漂白效果，从而能够在高放大倍数下进行光记录。
