Marek LeEtta J, Koskinen William C
Minnesota Department of Agriculture, 601 Robert Street North, St. Paul, MN 55155, USA.
J Agric Food Chem. 2007 Feb 7;55(3):571-6. doi: 10.1021/jf061440y.
Mice and rat populations are commonly controlled by two classes of rodenticide anticoagulants, coumarins and indandiones. However, poisoning of nontarget animals also often occurs. For cases such as these, a rapid, multiresidue method, which provides positive confirmation for both classes of anticoagulant rodenticides, is needed by diagnostic laboratories. A method was developed for the determination of seven anticoagulant rodenticides, coumafuryl, pindone, warfarin, diphacinone, chlorophacinone, bromadiolone, and brodifacoum, in diverse matrices, animal feed, cooked beef, and fruit-flavored beverages using high-performance liquid chromatography/electrospray/mass spectrometry. Detection was by MS/MS with electrospray ionization in negative mode. Confirmation was by retention time, m/z of molecular ion, and two parent-daughter transitions. Recoveries from selected the matrices ranged from 61 to 117%. Limits of quantitation were as low as 1.5-4.5 ng g-1. The developed method was rapid and provided the simultaneous confirmation and quantification of the seven anticoagulant rodenticides.
小鼠和大鼠种群通常由两类杀鼠剂抗凝血剂控制，即香豆素类和茚满二酮类。然而，非靶标动物中毒的情况也经常发生。对于此类案例，诊断实验室需要一种快速的多残留方法，能够对这两类抗凝血杀鼠剂进行阳性确证。已开发出一种方法，用于测定动物饲料、熟牛肉和果味饮料等多种基质中的七种抗凝血杀鼠剂，即呋杀鼠灵、茚满二酮、华法林、敌鼠、氯鼠酮、溴敌隆和溴鼠灵，采用高效液相色谱/电喷雾/质谱联用技术。检测采用负模式电喷雾电离的串联质谱法。通过保留时间、分子离子的质荷比以及两个母离子-子离子跃迁进行确证。从选定基质中的回收率在61%至117%之间。定量限低至1.5 - 4.5 ng g-1。所开发的方法快速，能够同时确证和定量这七种抗凝血杀鼠剂。
