高效液相色谱-离子阱质谱联用快速定性分析茚满二酮类灭鼠剂

[Rapid qualitative analysis of indandione rodenticides by high-performance liquid chromatography coupled with ion trap mass spectrometry].

作者信息

Chen Xiaohong, Cai Meiqiang, Jin Micong

机构信息

Ningbo Key Laboratory of Poison Research and Control, Ningbo Municipal Center for Disease Control and Prevention, Ningbo 315010, China.

出版信息

Wei Sheng Yan Jiu. 2010 Sep;39(5):586-90.

PMID:21033437

Abstract

OBJECTIVE

To establish a method for the simultaneous qualitative analysis of four indandione rodenticides by high-performance liquid chromatography coupled with ion trap mass spectrometry (LC-IT/MS).

METHODS

After the indandione rodenticides in bait were extracted by acetone, and cleaned by solid-phase extraction (SPE), the liquid chromatographic separation and the MS/MS cracking were performed in the optimal instrument conditions by LC-IT/MS. Qualitative detection were carried out by the principle of mass spectrometry analysis of three qualitative ions.

RESULTS

The MS/MS fragment ions had good characteristics and stability, the cracking ways to the parent ions and fragment ions were clear. Qualitative ion pairs were m/z 339-->167 and m/z 339-->145 for diphacine, m/z 373-->201 and m/z 373-->145 for chlorophacinone, m/z 229-->145 and m/z229-->187 for valone, and m/z 229-->172 and m/z229-->145 for pindone.

CONCLUSION

This established method is simple, accurate and reliable for the qualitative analysis of indandione rodenticides.

摘要

目的

建立高效液相色谱-离子阱质谱联用（LC-IT/MS）同时定性分析4种茚满二酮类杀鼠剂的方法。

方法

用丙酮提取饵剂中的茚满二酮类杀鼠剂，经固相萃取（SPE）净化后，在最佳仪器条件下用LC-IT/MS进行液相色谱分离和二级质谱裂解，依据3个定性离子的质谱分析原理进行定性检测。

结果

二级质谱碎片离子特征性和稳定性良好，母离子与子离子的裂解方式清晰。敌鼠的定性离子对为m/z 339→167和m/z 339→145，氯鼠酮的定性离子对为m/z 373→201和m/z 373→145，杀鼠酮的定性离子对为m/z 229→145和m/z 229→187，鼠得克的定性离子对为m/z 229→172和m/z 229→145。