O'Kane S L, Garimella V, Sivarajasingham N, Drew P J, Cawkwell L
Cancer Biology Proteomics Group, Postgraduate Medical Institute of the University of Hull in association with the Hull-York Medical School, University of Hull, Hull, UK.
J Clin Pathol. 2007 Feb;60(2):216-7. doi: 10.1136/jcp.2006.037275.
A robust method to facilitate rapid laser microdissection and pressure catapulting (LMPC) coupled with direct polymerase chain reaction (dPCR) to eliminate the need for extraction of DNA before a PCR-based assay is described. This sequential LMPC-dPCR method is rapid and decreases the number of processing steps, reducing the chance of tissue loss and contamination.
本文描述了一种强大的方法，该方法有助于快速进行激光显微切割和压力弹射（LMPC），并结合直接聚合酶链反应（dPCR），从而无需在基于聚合酶链反应（PCR）的检测之前提取DNA。这种连续的LMPC-dPCR方法速度快，减少了处理步骤的数量，降低了组织丢失和污染的可能性。
