de Andrade C F, Castello Branco Júnior A
Departamento de Zoologia do Instituto de Biologia da Universidade Estadual de Campinas, SP-Brasil.
Rev Saude Publica. 1991 Oct;25(5):367-70. doi: 10.1590/s0034-89101991000500007.
The use of wooden troughs on stream beds, artificially colonized by blackfly larvae, is proposed for larvicide evaluations. Mortality was recorded 3 or 4 hours after treatment. Larval susceptibility was also evaluated utilizing the LT50 criterion. In there field assays Simulium (C.) pertinax populations from the litoral of S. Paulo and Rio de Janeiro States were shown to be resistant to temephos, even when subjected to high concentrations. Vectobac 12 AS, a Bacillus thuringiensis var. israelensis product, was shown to be more potent against late instar larvae and efficient in concentrations higher than 7,200 ITU/l (10 min). The LT50 to 3,744 ITU/l (10 min) was calculated as 70.9 min.
建议使用由蚋幼虫人工定殖的河床木槽进行杀幼虫剂评估。处理后3或4小时记录死亡率。还利用半数致死时间标准评估幼虫易感性。在这些现场试验中，圣保罗州和里约热内卢州沿海的骚扰库蠓种群即使在高浓度下也显示出对双硫磷具有抗性。Vectobac 12 AS，一种苏云金芽孢杆菌以色列变种产品，对老龄幼虫显示出更强的效力，并且在浓度高于7200国际毒力单位/升（10分钟）时有效。3744国际毒力单位/升（10分钟）的半数致死时间计算为70.9分钟。
