Hwang S D, Shun Y J, Meng C L
Army General Hospital 804, Taiwan, ROC.
Zhonghua Ya Yi Xue Hui Za Zhi. 1991 Sep;10(3):88-97.
In order to establish and understand the in vitro human gingival cell culture system, this study presents newly developed and characterized primary culture cell types derived from human gingival tissues. Cell cultures were established from human gingival tissues by means of the explant technique and monolayer culture. Cells were studied under stable growth conditions and were characterized in terms of their morphology, Giemsa staining, anti-epithelial cytoskeletal staining, and proliferative parameters. At confluence, disoriented fibroblast cells formed the multilayered culture. The epithelial nature of the epithelioid cells was confirmed by staining for cytoplasmic keratin which is an exclusive epithelial cell protein. The growth curve and cell doubling time of the fibroblasts were evaluated. The results indicate that both epithelial cells and fibroblasts can be cultured from human gingival tissue. This technique provides us with a stable source of normal cells for further in-depth in vitro studies.
为了建立并了解体外人牙龈细胞培养系统，本研究展示了新开发并鉴定的源自人牙龈组织的原代培养细胞类型。通过外植体技术和单层培养从人牙龈组织建立细胞培养物。在稳定的生长条件下研究细胞，并根据其形态、吉姆萨染色、抗上皮细胞骨架染色和增殖参数进行表征。汇合时，无定向的成纤维细胞形成多层培养物。通过对细胞质角蛋白（一种独特的上皮细胞蛋白）进行染色，证实了类上皮细胞的上皮性质。评估了成纤维细胞的生长曲线和细胞倍增时间。结果表明，上皮细胞和成纤维细胞均可从人牙龈组织中培养出来。该技术为我们提供了稳定的正常细胞来源，用于进一步深入的体外研究。
