Sato T, Yagori A, Niwa M
Department of Preventive and Community Dentistry, School of Dentistry Nippon Dental University, Tokyo, Japan.
Pharmacol Toxicol. 1987 Nov;61(5):313-5. doi: 10.1111/j.1600-0773.1987.tb01826.x.
Sensitivity to fluoride of human gingival fibroblasts derived from six different normal tissues (donor age; 17-36 years, male and female) and a foetal tissue were studied by measuring cell growth and protein synthesis in vitro. Fibroblasts derived from four different human foetal lungs were also studied in order to compare them with the gingival cells. Two cell lines of gingival and lung fibroblasts were derived from the same foetus. All of the young adult and adult gingival cells grew slowly in the medium containing 1.32 mM fluoride, while foetal cell growth, including the foetal gingival cells, was completely inhibited. Both foetal cultures had faster cell replication rates and greater protein synthesis rates than those of the young adult or adult cultures. The fluoride sensitivity was lower in all young adult and adult gingival fibroblasts than in the foetal cells. However, "aged" foetal cultures obtained by serial passage showed lower fluoride sensitivity. These "aged" cells also had prolonged generation times. These findings indicate that although the mechanism is unclear, the low fluoride sensitivity of the young adult and adult gingival fibroblasts observed in this study are likely to be related to their slower rate of cell growth.
通过体外测量细胞生长和蛋白质合成，研究了源自六种不同正常组织（供体年龄17 - 36岁，男女皆有）以及一种胎儿组织的人牙龈成纤维细胞对氟化物的敏感性。还研究了源自四种不同人胎儿肺的成纤维细胞，以便与牙龈细胞进行比较。两种牙龈和成纤维细胞系源自同一个胎儿。所有年轻成人和成人牙龈细胞在含有1.32 mM氟化物的培养基中生长缓慢，而胎儿细胞生长，包括胎儿牙龈细胞，完全受到抑制。两种胎儿培养物的细胞复制率和蛋白质合成率均高于年轻成人或成人培养物。所有年轻成人和成人牙龈成纤维细胞的氟化物敏感性均低于胎儿细胞。然而，通过连续传代获得的“老化”胎儿培养物显示出较低的氟化物敏感性。这些“老化”细胞的世代时间也延长了。这些发现表明，尽管机制尚不清楚，但本研究中观察到的年轻成人和成人牙龈成纤维细胞对氟化物的低敏感性可能与其较慢的细胞生长速率有关。
