Anti-phosphotyrosine antibodies--obtaining and purification.
作者信息
Stefănescu M, Cremer L, Cristescu C, Matache C, Szegli G
机构信息
Cantacuzino Institute, Bucharest, Romania.
出版信息
Roum Arch Microbiol Immunol. 1991 Jul-Sep;50(3):187-92.
PMID:1726504
Abstract
Anti-phosphotyrosine antibodies were obtained by rabbits immunization using bovine serum albumin-phosphotyrosine complex. Hyperimmune rabbit serum was purified on affinity chromatography column obtained by phosphotyramine binding on Sepharose 4 B, CNBr-activated. The antibodies obtained after purification were tested by ELISA and immunoblotting.
摘要
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