Kamps M P
Methods Enzymol. 1991;201:101-10. doi: 10.1016/0076-6879(91)01012-q.
The techniques of detecting phosphotyrosine-containing proteins by AIA and purifying these proteins by affinity chromatography with anti-phosphotyrosine monoclonal antibodies are both reliable and consistent. Although not every tyrosine-phosphorylated protein can be detected and purified by using these techniques, a majority can. No doubt future studies will employ these approaches both for analyzing the specific role that tyrosine protein phosphorylation plays in regulating cell division and for investigating the properties of heretofore uncharacterized proteins that contain phosphotyrosine.
通过亲和免疫分析（AIA）检测含磷酸酪氨酸蛋白以及利用抗磷酸酪氨酸单克隆抗体通过亲和层析法纯化这些蛋白的技术既可靠又稳定。虽然并非每一种酪氨酸磷酸化蛋白都能通过这些技术检测和纯化出来，但大多数都可以。毫无疑问，未来的研究将采用这些方法来分析酪氨酸蛋白磷酸化在调节细胞分裂中所起的特定作用，以及研究迄今尚未鉴定的含磷酸酪氨酸蛋白的特性。
