Domanska-Janik Krystyna, Habich Aleksandra, Sarnowska Anna, Janowski Mirosław
NeuroRepair Department, Medical Research Center, Polish Academy of Sciences, ul. Pawinskiego 5, 02-106 Warsaw, Poland.
Acta Neurobiol Exp (Wars). 2006;66(4):279-91. doi: 10.55782/ane-2006-1617.
Human umbilical cord blood (HUCB) is considered a promising source of neural progenitors capable of being used for cellular therapies in neurological disorders. Here we review briefly our work on the elucidation of mechanisms and development of practical standards as regards the selection, maintenance and use of cord blood derivatives for such purposes. Our results join those of other recent studies in suggesting strongly that, the generation of neural-like cells from tissue belonging to a different germ layer (such as a cord blood is) is most probably explained by reference to a discrete subpopulation of embryonic-like stem cells of pluripotent characteristics. Such cells identified in cord blood through their expression of specific genetic and protein markers can be expanded in vitro and directed toward neurally-committed progenitors differentiating further into more mature neuron-like or macroglia-like cell phenotypes. From this HUCB-derived neural progenitor fraction a novel neural-like stem cell line (HUCB-NSC) has been developed, and characterized in respect of in vitro and in vivo (post-transplantation) properties.
人脐带血（HUCB）被认为是一种有前景的神经祖细胞来源，可用于神经系统疾病的细胞治疗。在此，我们简要回顾一下我们在阐明相关机制以及制定关于为此目的选择、维持和使用脐带血衍生物的实用标准方面所开展的工作。我们的结果与其他近期研究结果一致，强烈表明，从属于不同胚层的组织（如脐带血）中产生神经样细胞，很可能是由于存在具有多能特性的离散的胚胎样干细胞亚群。通过特定基因和蛋白质标志物的表达在脐带血中鉴定出的此类细胞可在体外扩增，并定向分化为神经定向祖细胞，进一步分化为更成熟的神经元样或大胶质细胞样细胞表型。从这种源自脐带血的神经祖细胞部分中，已开发出一种新型神经样干细胞系（HUCB-NSC），并对其体外和体内（移植后）特性进行了表征。
