Habich Aleksandra, Domanska-Janik Krystyna
NeuroRepair Department, Medical Research Institute, Polish Academy of Sciences, Warsaw, Poland.
Acta Neurobiol Exp (Wars). 2011;71(1):1-11. doi: 10.55782/ane-2011-1818.
Human umbilical cord blood (HUCB) has been established as a promising source of hematopoietic as well as various non- hematopoietic stem cell populations. This offers numerous advantages of HUCB stem/progenitor cells for therapies, however in vitro conditions that contribute to long term propagation of proliferating undifferentiated cells have not yet been established. Here we evaluate culture conditions promoting spheroid aggregates/neurospheres formation which, together with serum withdrawal and mitogenes treatments in strictly defined media, maintain population of HUCB progenitor cells in undifferentiated and dividing state exhibiting neurogenic potential in vitro. Our results indicate that formation and maintenance of three-dimensional aggregates enhanced by cell culture rotating motion, is crucial for high and prolonged expression of genes and proteins characteristic for cord blood stem cells and their further neural commitment.
人脐带血（HUCB）已被确认为造血干细胞以及各种非造血干细胞群体的一个有前景的来源。这为HUCB干/祖细胞用于治疗提供了众多优势，然而，有助于增殖未分化细胞长期增殖的体外条件尚未确立。在此，我们评估促进球体聚集体/神经球形成的培养条件，这些条件与在严格定义的培养基中血清去除和有丝分裂原处理一起，使HUCB祖细胞群体维持在未分化和分裂状态，在体外表现出神经发生潜能。我们的结果表明，细胞培养旋转运动增强的三维聚集体的形成和维持，对于脐带血干细胞特征性基因和蛋白质的高表达及长期表达以及它们进一步的神经定向分化至关重要。
