Feirabend H K, Ploeger S
Dept. of Physiology, University of Leiden, The Netherlands.
Eur J Morphol. 1991;29(3):185-97.
The quality of microwave adaptations of three classical neuroanatomical staining methods (the Nissl, Klüver-Barrera and Häggqvist stains) was tested on frozen serial sections from human brain specimens which has been stored for up to 10 years in 10% formalin. The conclusion was that the use of microwave irradiation reduces processing time and/or concentrations of the chemicals used, whereas the light microscopical quality of the stains considered is equal or improved as compared to their original counterparts. Next, a comparison was made between microwave adapted stains and classical procedures, which, except for the use of a conventional oven as heat source together with pre-heated solutions, were entirely identical. It appeared, that at light microscopical level no difference can be appreciated between the effect of internally (using microwave irradiation) and externally (using a conventional oven) supplied heat on the staining result.
在取自人类脑标本的冷冻连续切片上，对三种经典神经解剖学染色方法（尼氏染色、克吕弗-巴雷拉染色和哈格奎斯特染色）的微波适配质量进行了测试，这些脑标本已在10%福尔马林中保存长达10年。结论是，使用微波辐射可减少处理时间和/或所用化学试剂的浓度，而所考虑的染色的光学显微镜质量与原始对应物相比相当或有所提高。接下来，对微波适配染色法与经典方法进行了比较，除了使用传统烤箱作为热源并搭配预热溶液外，二者完全相同。结果表明，在光学显微镜水平上，内部（使用微波辐射）和外部（使用传统烤箱）供热对染色结果的影响没有差异。
